从活性污泥中提取聚-β-羟基丁酸酯的方法和条件的研究  

Study on extraction method and condition of poly-β-hydroxybutyrate(PHB) from activated sludge

作　　者：刘哲君[1] 赵敏[1] 潘俊波[1,2] 钱永雨[1] 

机构地区：[1]东北林业大学生命科学学院,黑龙江哈尔滨150040 [2]东北农业大学农学院,黑龙江哈尔滨150030

出　　处：《现代化工》2009年第S2期214-217,共4页Modern Chemical Industry

摘　　要：考察了提取方法和提取条件对从活性污泥中提取聚-β-羟基丁酸酯(PHB)效果的影响。结果表明,利用EDTA、SDS和NaClO混合组成的破壁溶液同有机溶剂氯仿边破壁边提取的方法最为有效。在该方法中,当EDTA溶液、SDS溶液和NaClO的体积比为5∶5∶9、污泥干重与总破壁剂体积(g/mL)之比为0.29%时,在35℃下提取7 h可达最佳提取效果,此时活性污泥内PHB提取率达75.42%。The effects of extraction method and condition of poly-β-hydroxybutyrate from activated sludge were studied.The results show that when using dispersion of DETA,SDS,sodium hypochlorite solution and chloroform synchronously,the highest extraction rate is achieved.The optimal extraction conditions are the activated sludge being extracted in chloroform with 0.29%(w/v) of mixed solution [V(EDTA)∶V(SDS)∶V(NaClO)=5∶5∶9] at 35℃ for 7 h.At these conditions,the extraction rate can reach 75.42%.

关 键 词：聚-β-羟基丁酸酯(PHB) 活性污泥 提取 

分 类 号：X703.1[环境科学与工程—环境工程]