玉米DNA导入水稻的RAPD分子验证  

Molecular Verification of Maize DNA Introduced into Rice

作　　者：王永斌[1] 孟庆勇[1] 刘传雪[2] 王广金[1] 

机构地区：[1]黑龙江省农业科学院生物技术研究所,哈尔滨1500862 [2]黑龙江省农业科学院佳木斯水稻研究所,佳木斯154026

出　　处：《生物技术通报》2009年第S1期136-138,150,共4页Biotechnology Bulletin

基　　金：黑龙江省攻关项目GB07B104(2007-2009)

摘　　要：对高光效C4植物玉米DNA为供体、通过花粉管通道技术导入水稻所获得的水稻后代进行了RAPD分析.结果表明:从60个引物中找到12个引物检测出DNA的多态性,证明外源DNA导入受体后引起后代基因组的显著变异.Offspring of rice derived from introduction of high photosynthetic efficiency maize DNA through pollen tube channel was verified in this paper.The result showed that there were polymorphism between offsprings and recipient detected by 12 primers out of the 60 arbitrary primers.This indicated that variation of offspring in genomic DNA was induced by the introduction of exogenous DNA into recipient.

关 键 词：玉米 外源DNA导入 水稻 RAPD 

分 类 号：S511[农业科学—作物学]