布氏罗非鱼微卫星位点筛选和群体遗传多样性分析  

Isolation of Microsatellite DNA and Genetic Diversity Analysis on Tilapia buttikoferi

作　　者：牟希东[1] 李小慧[1] 胡隐昌[1] 汪学杰[1] 宋红梅[1] 罗建仁[1] 

机构地区：[1]中国水产科学研究院珠江水产研究所,广州510380

出　　处：《生物技术通报》2009年第S1期236-241,共6页Biotechnology Bulletin

基　　金：中国水产科学研究院水产种质资源与养殖技术重点开放实验室开放基金项目(2006A007);农业部农作物病虫鼠害疫情监测与防治项目(2130108)

摘　　要：为阐明布氏罗非鱼(Tilapia buttikoferi)群体遗传变异和遗传结构状况,采用50个尼罗罗非鱼特异性的微卫星分子标记对45个布氏罗非鱼个体进行遗传检测。结果有27对引物能获得稳定的特异性条带,占总数的54%,其中16个多态性微卫星座位共检测出52个等位基因,每个座位的等位基因数为2～6之间,平均每个座位为3.24;平均观测杂合度(Ho)为0.5266,平均期望杂合度(He)为0.5237,平均多态信息含量为0.4652,表明布氏罗非鱼群体遗传多样性较丰富,种群结构处于合理状态。To assess its genetic variability and population structure further,50 Oreochromis niloticus microsatellite loci were analyzed for a total of 45 Tilapia buttikoferi individuals.27 of 50(54%) sucessfully were amplified in Tilapia buttikoferi and a total of 52 alleles were detected across the 16 polymorphic loci assayed.The number of alleles varied from two to six,giving a mean number of 3.24 alleles.The average observed heterozygosity(Ho),average expected heterozygosity(He) and polymorphism information content(PIC) were 0.5266,0.5237 and 0.4652 respectively.These results showed that Tilapia buttikoferi bears a high genetic diversity and a reasonable population structure.

关 键 词：布氏罗非鱼 微卫星标记 遗传多样性 

分 类 号：S917.4[农业科学—水产科学]