缘管浒苔Rubisco酶大亚基基因编码序列rbcL克隆及分析  

Cloning and Sequence Analyzing of the rbcL Gene from Ulva linza

作　　者：尹顺吉[1] 应成琦[1] 汤文仲[1] 张婷[1] 何建华[1] 何培民[1] 

机构地区：[1]上海海洋大学水产与生命学院农业部水产种质资源与养殖生态重点开放实验室,上海201306

出　　处：《生物技术通报》2009年第S1期266-270,共5页Biotechnology Bulletin

基　　金：国家自然科学基金(30371101);教育部博士点基金;上海市教委优势(重点)学科资助项目(S30701)

摘　　要：主要对缘管浒苔光合作用第一关键酶Rubisco大亚基基因(rbcL)进行了克隆分离。首先通过PCR特异性扩增叶绿体基因编码的缘管浒苔大亚基编码序列rbcL部分基因序列(1 035 bp)。依据基因步移原理,首次克隆得到缘管浒苔rbcL5′上游非翻译区序列(224 bp)。据推测,rbcL 5′上游非翻译区序列存在类似原核生物的启动子元件-10区(TAAAAT)和-35区(TTGAAA)。此外,依据3′-RACE(cDNA末端快速扩增技术)原理,克隆得到缘管浒苔rbcL3′末端cDNA序列(579 bp)。In this paper,the sequence of Rubisco,the first key enzyme of photosynthesis in Ulva linza,was cloned.Firstly,The sequence of the chromosomally encoded Rubisco large subunit gene(rbcL) of Ulvz linza was cloned(1035 bp).According to gene walking method,Ulva linza rbcL 5'-untranslated region was cloned for the fint time(224 bp).The sequence was predicted to have-10 box(TATTAA)and-35 box(TTGAAA) similar to prokaryotic promoter motif.In addition,3'-end of rbcL cDNA(579bp) was cloned by 3'-RACE(rapid amplification of cDNA ends).

关 键 词：RBCL 缘管浒苔 基因步移 启动子 

分 类 号：Q943.2[生物学—植物学]