使用硅藻土处理发酵废液、提高丁醇发酵废液回用率  

Increase of Butanol Fermentation Residual Recycle Ratio with the Aid of Treating Waste Ssupernatant by Silicalite

作　　者：马兴[1] 张书敏[1] 冯豪杰[1] 段生兵[1] 史仲平[1] 

机构地区：[1]江南大学生物工程学院工业生物技术教育部重点实验室,无锡214122

出　　处：《生物技术通报》2009年第S1期367-372,共6页Biotechnology Bulletin

基　　金：国家重点基础研究发展规划项目("973项目")(2007CB714303)

摘　　要：以生物柴油为萃取剂耦联丁醇发酵,能以最为节能的方式生产"改良型"生物柴油、提高发酵性能,但却也产生了大量发酵废液、萃余液回用率超过50%次轮发酵就无法正常进行。探计了使用硅藻土处理发酵废液、提高废液回用率的可能性和最适条件,在使用40目硅藻土、用量3%(w/v)的条件下,废液回用率可以提高至75%;对提高废液回用率的原因进行了初探,利用少量硅藻土可以吸附发酵残液中的丁醇,减少因美拉德反应所生成的不同分子量的增殖抑制型色素物质。原位添加微量硅藻土有利于丁醇发酵的进行,在使用油醇的萃取发酵条件下,丁醇总生产强度提高了8%。Acetone-butanol(AB) extractive fermentation with biodiesel originated from waste cooking oil as the extractant,could enhance the fermentation performance and manufacture'property-improved biodiesel'in the most energy-saving mode.However,this process also produces a large amount of waste supernatant,and fermentation residual recycle ratio over 50%is impossible.Aiming at increasing fermentation residual recycle ratio to save the valuable water resources,in this study,the possibility of using silicalite to enhance waste supernatant utilization ratio and the relevantly optimal condition were investigated.Under the condition of using 40 mesh and 3% (w/v) silicalite for waste supernatant pre-treatment,the waste supernatant recycle ratio could increase from 50%to 75%without fermentation performance deterioration.The reason for waste supernatant recycle ratio enhancement with the aid of silicalite pre-treatment was also preliminarily investigated.Silicalite effectively absorbs butanol in the waste supernatant so that inhibitory pigment formation caused by Millard reaction could be potentially reduced.In-situ addition of tiny amount of silicalite would further enhance AB fermentation performance,and the fermentation productivity could increase 8%for the case of extractive AB fermentation with olyel alcohol as the extractant.

关 键 词：萃取剂 丁醇 发酵 废液回用 

分 类 号：X703[环境科学与工程—环境工程]