提高布氏白僵菌产孢量的培养基及培养条件研究  

Study on Medium and Cultivation Conditions to Increase Spore Yield of Beauveria brongniartii

作　　者：李茂业[1] 林华峰[1] 刘苏[1] 李世广[1] 

机构地区：[1]安徽农业大学植物保护学院,合肥230036

出　　处：《生物技术通报》2009年第S1期380-383,共4页Biotechnology Bulletin

基　　金：国家自然科学基金重点项目(30330500);国家农业行业科研专项(03_200803003)

摘　　要：采用液固两相培养法,筛选出了适用于培养布氏白僵菌Bbr84菌株的培养基,并测定了培养基、培养时间和光照条件对产孢量的影响。结果表明:用液固双相培养法培养布氏白僵菌获取高孢粉,在液相培养阶段,SDAY+2%麦芽糖是较好的培养基;固相培养阶段,以大米+稻壳+黄粉虫粪组合产孢量较高,且在培养的第五天白僵菌产孢量即达到高峰,这比已报道的其他培养料配方的产孢高峰时间缩短2-3天。培养前期,应以黑暗环境为主,以增强菌丝生长,但后期应适当供给光照,以促进菌丝大量产孢。Using the Liquid-solid culture medium to select the suitable medium culture for the strain of Bbr84 and determine the spore yield of different medium,different time and different illumination of development.The results show that using liquid and solid media fermentation to culture Beauveria brongniartii Petch and obtain spores.SDAY supplied with 2%malt sugar was good liquid medium in the period of liquid culture.During the course of solid-phase culture,The solid culture medium of boiled rice mixed with the excrement of Tenebrio molitor L.and rice husk produced the most spore,and the peak of spore yield was 5 days after culture,2-3 days was decreased according to former report..It should mainly give dark environment in order to increase hyphal growth in early period culture,however,the latter period should be adequate light so as to promote a large number of spore yield.

关 键 词：布氏白僵菌 液固双相培养 培养技术 产孢量 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]