组织多普勒成像评价大鼠左心室舒张功能的增龄性改变  

Assessment of age-related changes in left ventricular diastolic function in rats by tissue Doppler imaging

作　　者：曹丽[1] 林美光[2] 王佩显[2] 周欣[3] 陈兴[2] 张丽[2] 

机构地区：[1]天津医科大学总医院老年病研究室,天津300052 [2]天津医科大学总医院心血管内科,天津300052 [3]天津武警医学院附属医院心血管病研究所心内科,天津300162

出　　处：《中国医学影像技术》2009年第S1期8-10,共3页Chinese Journal of Medical Imaging Technology

摘　　要：目的利用组织多普勒成像(TDI)技术评价从幼年到老年不同月龄的正常Wistar大鼠心脏舒张功能的增龄性改变。方法选用月龄分为1、2、5、12、20个月的正常雄性Wistar大鼠60只,分别行经胸超声心动图检查,最后处死动物取左心室称重并送组织病理学检测。结果随增龄各组大鼠左心室相对质量(LVRM)明显增加(P<0.01),于20个月增至最重。超声所计算的LVRM与实体标本所测值呈正相关(r=0.780,P<0.001)。二尖瓣口舒张末期血流速度(E)20月龄组与各低龄组相比较组间无统计学差异(P>0.05),二尖瓣环舒张早期最大运动速度(Em)随月龄增长逐渐下降,二尖瓣环舒张晚期最大运动速度(Am)峰值呈上升趋势,Em/Am比值随月龄增长逐渐下降,Em/Am比值在1～5个月龄组>1,12个月龄和20个月龄组<1。组织病理学检测:20个月龄组心肌出现灶性纤维化。结论DTI技术能够检测大鼠左心室舒张功能和观察其增龄性改变的规律,为基础实验研究提供可靠的无创方法及依据。Objective To evaluate the age-related changes in left ventricular diastolic function on various age Wistar rats with tissue doppler imaging(TDI).Methods Sixty male Wistar rats divided into 5 groups(aged 1,2,5,12 and 20 months were enlisted,each group n=12).All groups were examined in echocardiography.Finally,histopathologic examination was performed.Results Left ventricular myocardial weight of rats increased with aging(P<0.01),and the heaviest was in 20 months of age.There was positive correlation between left ventricular mass examined by echocardiography with that examined by necropsy(r=0.803,P<0.001);the maximal early diastolic velocities(Em)of mitral annulus and Em/Am ratio were significantly decreased by aging(P<0.01),however,the maximal later diastolic velocities(Am)of mitral annulus has a tendency to increase with aging.Em/Am ratio>1 at 1,2,and 5 months group,conversely Em/Am ratio<1 at 12 and 20 months group.Histopathologic study demonstrated focal myocardial fibrosis in 20 months group.Conclusion TDI is a reliable non-invasive method which can substitute the invasive hemodynamic method to evaluate age-associated changes in rat's left ventricular diastolic function.

关 键 词：组织多普勒成像 大鼠 左心室舒张功能 增龄 

分 类 号：R541[医药卫生—心血管疾病]