整株转化法及其在油菜上的应用与展望  

Research in In Planta Transformation Methods and Their Applications and Prospects in Brassica napus

作　　者：邹智[1] 卢长明[1] 

机构地区：[1]中国农业科学院油料作物研究所基因工程与转基因安全研究室,武汉430062

出　　处：《植物学报》2009年第2期236-244,共9页Chinese Bulletin of Botany

基　　金：国家自然科学基金(No.30471009);863计划(No.2008AA10Z152)

摘　　要：近年来,在植物遗传转化和功能基因组学研究的迫切需求下,一些不依赖于受体细胞脱分化、再分化过程的转化技术(即所谓的整株转化法)相继出现,它们大多以分生组织或生殖细胞作为受体,不受基因型限制,具有操作简单、转化效率高、无体细胞变异和后代遗传稳定等优点。该文对整株转化法的最新研究进展进行综述,并重点讨论了其在油菜(Brassica napus)上的应用现状与前景。In planta transformation methods for obtaining transgenic plants without dedifferentiation and redifferentiation of receptor cells have been explored because of their advantages of genotype independence,ease of operation,high efficiency,lack of somatic mutation,and genetic stability through future generations.Most methods use meristematic tissues or reproductive cells as receptors.This review focuses on the current progress of in planta transformation methods,with an emphasis on their applications and prospects for genetic transformation and functional genomics research in Brassica napus.

关 键 词：根癌农杆菌 油菜 整株转化法 分生组织 生殖细胞 

分 类 号：S565.4[农业科学—作物学]