不同剂量葛根素在大鼠体内的药代动力学  

Comparison of Pharmacokinetics between Different Doses of Puerarin in Rats

作　　者：李煦颖[1] 张懋璠 赵妍[1] 王欣[1] 孟胜男[1] 

机构地区：[1]中国医科大学药学院药剂学教研室,沈阳110001

出　　处：《中国医科大学学报》2009年第12期885-887,共3页Journal of China Medical University

基　　金：辽宁省自然科学基金资助项目(20082103)

摘　　要：目的比较不同给药剂量下葛根素在大鼠体内的药代动力学。方法大鼠分别灌胃给予低、中、高剂量(250、500、750mg·kg-1)的葛根素,按实验设计在不同时间点采血,应用高效液相色谱法测定葛根素血药浓度,采用DAS2.0软件计算药动学参数。结果葛根素在大鼠体内药动学过程均符合二室模型;低、中、高3个剂量组的Tmax、MRT均无显著性差异;中剂量组t1/2z与高剂量组、低剂量组均有显著性差异;Vz/F、CLz/F与剂量基本呈线性关系;在250～500mg·kg-1剂量内,AUC与剂量基本呈线性关系,剂量增加至750mg·kg-1时,AUC无明显增加。结论不同给药剂量的葛根素在大鼠体内药动学过程存在差异,给药剂量在250～500mg·kg-1内,葛根素体内药动学行为呈线性关系,给药剂量在500～750mg·kg-1内,葛根素的药动学行为呈非线性关系。Objective To compare the pharmacokineics of Puerarin in rat after oral administration of different doses. Methods Rats received 250,500,750 mg·kg-1 of Puerarin by oral administration respectively. We took blood in different time according to the experimental design.The plasma concentration of the Puerarin was analyzed by HPLC. The pharmacokinetics parameters was calculated with DAS 2.0. Results The pharmacokinetics of Puerarin at differernt doses were all best fitted with two-compartment models in rats after oral administration. There were no significant differences in pharmacokinetic parameter Tmax and MRT among the three doses,but Vz/F,CLz/F have sig- nificant differences in the three doses. AUC increased with dose rising at 250～500 mg·kg-1,but there was no significant increasing at 750 mg·kg-1. Conclusion The pharmacokinetics of Puerarin at the three doses were different and showed non-linear pharmacokinetics in rats.

关 键 词：葛根素 药代动力学 高效液相色谱法 

分 类 号：R285.5[医药卫生—中药学]