雷公藤粗提物毒力测定及甲素含量分析  

Toxicity test of crude extracts from Tripterygium wilfordii and content analysis of triptolide

作　　者：黄彬彬[1] 李春维[1] 吴刚[1] 

机构地区：[1]福建农林大学植物保护学院,福建福州350002

出　　处：《生物安全学报》2008年第3期163-167,共5页Journal of biosafety

基　　金：科技部国际合作项目(05DFA30440);福建省科技厅重点项目(2009N0018)

摘　　要：以无水乙醇为溶剂从雷公藤根皮中提取有效成分,用高效液相色谱法分析雷公藤粗提物中甲素含量,并将雷公藤粗提物配成5%的乳油,用浸叶法和药膜法测定小菜蛾二龄幼虫、烟粉虱成虫以及无翅成蚜的室内毒力,并以几种杀虫剂作为对比。结果表明:该批雷公藤粗提物中甲素含量为0.0012%;5%雷公藤粗提物乳油对小菜蛾二龄幼虫、烟粉虱成虫以及无翅成蚜具有一定的生物活性,但与其他几种常用杀虫剂相比活性较低,还需对其进一步精制和提纯。The effective components were extracted from root bark of Tripterygium by using anhydrous alcohol as the solvent,determinating the content of triptolide in crude extracts from Tripterygium by HPLC and the bioactivities comparision of several insecticides and self-made 5%EC of crude extracts from Tripterygium against 2^(nd)-instar larvae of Plutella xylostella,adult Bemisia tabaci and apterous adult aphid Lipaphis erysimi with leaf dipping method and residual film.Experimental results were as follows.The content of this batch of Triptolide in crude extracts from Tripterygium was 0.0012%.5%EC of crude extracts from Tripterygium has a certain biological activity on 2^(nd)-instar larvae of P.xylostella,adult B.tabaci and apterous adult aphid,but the biological activity was lower than other several insecticides.So the crude extracts from tripterygium should be refined and purified.

关 键 词：雷公藤 粗提物 毒力 甘蓝蚜 小菜蛾 烟粉虱 甲素 

分 类 号：S482.3[农业科学—农药学]