抗HCV-NS3法检测肝组织中丙型肝炎病毒抗原的研究  

Detection of HCV Antigen-NS3 in Liver Tissue with Monoclonal ANTI-HCV by Direct Enzyme Immuno-assay

作　　者：李钦伟[1] 马京香[1] 

机构地区：[1]山东省血液中心,济南250014

出　　处：《医学检验与临床》2008年第1期6-8,共3页Medical Laboratory Science and Clinics

摘　　要：以单克隆抗-HCV-NS3直接酶标法对石蜡包埋肝组织中丙肝炎病毒抗原(HCAg-NS3)进行测定,该抗体系应用基因重组表达丙肝抗原(HCV-NS3区-C33-C)免疫小鼠并通过细胞融合术后获得。49例病毒性肝炎患者肝组织测定结果,抗-HCV阳性组的HCAg-NS3检出率为51.9%(14/27),抗-HCV阴性组为13.6%(3/22),两组有显著性差异(P<0.01),以慢性重症肝炎和肝硬化患者检出率最高。HCAg-NS3阳性物在肝细胞的胞核或胞浆中均可见呈棕黄色细小颗粒状,以核型居多。直接酶标法测定HCAg与原位杂交法测定HCV RNA的比较,其符合率为81.6%(40/49)。本项技术具有简便、快捷、特异性、敏感性佳、图象清晰等优点,易于推广应用,为HCV感染的临床和发病机理研究提供重要检测手段。We developed a direct cnzymc immune-assay for the detection of HCV antigen-NS3 in forma-lin-paraffin-embedded liver tissue from 49 viral hepatitis patients.The monoclonal anti-HCV-NS3 was obtained from recombinant HCAgNS3-C33-C.It has been proveo that this a rapid,simple,specific and sensitive method.In all of 49 cases with viral hepatitis,the detective rate of HCV-NS3 antigen was 34.7%(17/49),51.9%(14/27)in anti-HCV postive patients,and 13.6%(3/12) in anti-HCV negative patients.The posi-rive stain was found more on the nucleus than that in cytoplasm.Hepatocytes with posluve Staining distributed sporadically within the lobule.Confirmation tests of this method showed 1uhat this tech-nique wss quite specific.This result was compared with in situ hybridization detection of HCV RNA and the corresponsible rate was 81.6%(P>0.05).The influence of HBV repdcaion during HCV/HBV double infection was dlsscused.

关 键 词：丙型肝炎病毒 直接酶标法 单克隆抗HCV-NS3 

分 类 号：R446.6[医药卫生—诊断学]