HIV病毒对温度敏感性的实验研究  

Study on the Sensitivity of HIV to Different Temperatures

作　　者：袁霖[1] 马丽英[1] 邓素英[1] 纪燕[1] 邵一鸣 

机构地区：[1]中国疾病预防控制中心性病艾滋病预防控制中心,北京100050

出　　处：《中国病毒病杂志》2008年第2期95-97,共3页Chinese Journal of Viral Diseases

摘　　要：目的观察HIV病毒对不同温度的敏感性,为HIV消毒和灭活提供实验依据。方法制备HIV-1SF33病毒上清,分别放置在4℃冰箱,25℃、37℃、56℃和70℃水浴中,间隔一定时间后取样测定病毒滴度(TCID50)。结果HIV-1SF33滴度在4℃、25℃和37℃下分别于12h、6h和4h后出现10倍左右的降低,且随着时间的延长,HIV-1SF33滴度下降趋势越为明显;随着温度的升高,HIV-1SF33滴度随之下降,4℃和25℃条件下,HIVSF33放置24h,滴度分别下降2.7log和3.7log,37℃条件下放置24h,病毒丧失感染性;56℃作用10min或70℃作用2min,HIV病毒被迅速灭活,已检测不出活病毒。结论HIV病毒对热敏感,但在4℃和25℃条件下,液体环境中的HIV病毒可存活一段时间,应重视冷藏和常温下HIV污染物的消毒。热处理是灭活HIV病毒的有效方法。Objective To study the sensitivity of HIV to different temperatures and offer laboratory evidence for HIV disinfection.Methods HIV-1SF33 virus supernatant fluid samples were prepared and exposed to 4℃,25℃,37℃,56℃ and 70℃ for different period of time.The infectivity of these samples(TCID50)was examined.Results TCID50 of HIV-1SF33 showed 10-fold decrease after exposure to 4℃ for 12 h,25℃ for 6 h and 37℃ for 4 h,respectively.The longer the sample was exposed,the lower the TCID50 was.Along with the rising of the temperature,TCID50 of HIV-1SF33 decreased.After 24 h of exposure to 4℃ and 25℃,TCID50 of HIV-1SF33 decreased 2.7 log and 3.7 log,respectively.After staying at 37℃ for 24 h,56℃ for 10 min or 70℃ for 2 min,HIV-1SF33 lost its infectivity.Conclusion HIV in suspension can survive for a period of time at 4℃ or 25℃.Therefore the disinfection of HIV positive liquids and contaminants stored in freezer or normal temperature should be emphasized by lab staffs.HIV is very sensitive to heat.Heating is an effective method to inactive the virus.

关 键 词：HIV 温度 敏感性 滴度 

分 类 号：R373[医药卫生—病原生物学]