乙肝病毒核心抗原DNA疫苗诱导小鼠抗原特异性CD8^+T细胞动态变化和动态分布  

Direct ex vivo kinetic and distribution analysis of CD8^+T cells responses induced by hepatitis B virus core gene DNA vaccine

作　　者：张炜[1] 董生福[1] 孙淑惠[1] 汪垣[2] 李光地[2] 瞿涤[1] 

机构地区：[1]复旦大学上海医学院卫生部分子病毒重点实验室,上海200032 [2]中国科学院上海生化细胞研究所,上海200032

出　　处：《中国免疫学杂志》2004年第6期379-383,共5页Chinese Journal of Immunology

基　　金：国家 973项目 (G19990 5 410 5 );国家自然科学基金项目 ( 3 0 0 70 693 )

摘　　要：目的 :研究乙肝病毒核心抗原 (HBcAg)DNA疫苗诱导的细胞免疫应答。方法 :用表达乙肝病毒核心抗原N端1 4 4个氨基酸的重组质粒DNA(简称pHBc1 4 4 ) 1 0 0 μg免疫C5 7BL 6小鼠 ,用细胞内细胞因子染色技术检测小鼠体内抗原特异性CD8+ T细胞的动态变化。结果 :pHBc1 4 4免疫后抗原特异性CD8+ T细胞逐渐增多 ,在初次免疫的第 1 4天出现第一个免疫应答高峰 ,此后抗原特异性CD8+ T细胞数量逐渐下降进入免疫记忆期并在 1年内维持在稳定水平。加强免疫后在第 1 0天抗原特异性CD8+ T细胞数量达到高峰 ,约是初次免疫应答高峰的 2倍 ,此后抗原特异性CD8+ T细胞数量逐渐下降 ,但下降的速度比初次免疫应答减缓。结论 :pHBc1 4Objective:To study immune responses to HBV core gene DNA vaccines.Methods:One hundred micrograms of DNA from a recombinant expression plasmid,harboring DNA encoding the 144 amino acids of the N-terminus of HBV core gene(abbreviated pHBc144) was used to immunize C57BL/6 mice.The antigen-specific CD8 +T cell (CD8 +IFN-γ + T cells) were detected by intracellular cytokine staining.Results:After immunized by pHBc144 DNA vaccine the antigen-specific CD8 +T cell of mice increased gradually,the first peak of immune response appeared on 14 days later,then the number of antigen-specific CD8 +T cell decreased gradually and maintained in a steady level within one year.And after boosting the number of antigen-specific CD8 +T cell reached the second peak on 10 days later,the value is double,then the number of antigen-specific CD8 +T cell decreased slowly.Conclusion:HBcAg gene DNA vaccine pHBc144 could induce cellular immune responses successfully.

关 键 词：乙肝病毒核心抗原 DNA疫苗 

分 类 号：R510.3[医药卫生—内科学]