成骨细胞在快速成型技术构建的骨支架材料中粘附的实验研究  

Experimental Study of Periosteal Osteoblasts Adhesion to Artificial Bone Scaffolds Based on Rapid Prototype

作　　者：鲜苏琴[1] 柴枫 赵铱民 王忠义 刘小芳 李涤尘[3] 

机构地区：[1]口腔生物医学工程教育部重点实验室,四川大学,四川成都610041 [2]第四军医大学口腔医院修复科,陕西西安710032 [3]西安交通大学先进制造技术研究所,陕西西安710049

出　　处：《华西口腔医学杂志》2004年第3期248-251,共4页West China Journal of Stomatology

基　　金：国家自然科学基金资助项目 (599750 74)

摘　　要：目的　观察成骨细胞在用快速成型技术构建的具有不同孔隙率的骨支架材料中的粘附情况以及材料的孔隙率对成骨细胞粘附的影响 ,以探索用快速成型技术构建骨组织工程支架材料的可行性。方法　体外分离培养、扩增乳鼠颅骨骨膜成骨细胞 ,经传代培养作为种子细胞 ,接种于由快速成型技术构建 ,孔隙率分别为 80 %、90 %、95 %的骨支架材料中 ,复合培养 4d及 10d后 ,采用细胞计数及扫描电镜方法检测支架材料中细胞粘附的情况。结果　①各孔隙率组粘附成骨细胞总数均呈现随培养时间延长而增高的趋势 (P <0 0 5 ) ,粘附细胞数的增加量则以 80 %孔隙率组为最低 (0 35× 10 5个 ) ,而 90 %孔隙率组为最高 (2 84× 10 5个 ) ;②电镜观察发现用快速成型技术构建的骨支架材料具有良好的细胞相容性 ,成骨细胞能够在各孔隙率组支架材料内部粘附、聚集。结论　快速成型技术构建的骨支架材料为组织工程骨支架材料的设计、成型开辟了一条崭新途径。Objective To study the biocompatibility of bone engineering scaffolds designed and fabricated by CAD and Rapid Prototyping techniques.Methods Infant rat calvarias osteoblasts were isolated and expanded in vitro and the cells (2nd passage) were seeded onto scaffolds with porosity 80%,90%,95% at a density of 2.06×10+9/L. Cell adhesion number and morphology were measured with SEM after 4 days, 10 days co-culture.Results ①The osteoblasts′ adhesion amounts increased with culture time in three porosity group (P<0.05), but the increase were different among three groups, 80% group was 0.35×10+5, 90% group was 2.84×10+5;②Through SEM observations,it showed that osteoblasts adhered to all scaffolds well.Conclusion The scaffolds designed and fabricated by CAD and rapid prototyping own a good cellular biocompatibility. The results suggest the feasibility of using such scaffold fabricating method for bone tissue engineering research and clinical therapy.

关 键 词：骨组织工程 快速成型技术 细胞相容性 成骨细胞 

分 类 号：R318.08[医药卫生—生物医学工程]