抗咽喉细菌复合抗原的IgY抗体活性研究  

Study on the activity of IgY against complex bacteria in pharynx and throat

作　　者：蒙永祥 张玲[2] 黄春龙 林学颜[3] 

机构地区：[1]广东省大日生物化学药业有限公司,广州510507 [2]中山大学肿瘤防治中心实验研究部,广州510089 [3]中山大学中山医学院免疫学教研室,广州510089

出　　处：《中国免疫学杂志》2004年第6期393-395,共3页Chinese Journal of Immunology

摘　　要：目的 :应用咽喉分离的细菌混合抗原免疫母鸡 ,提取鸡蛋黄中IgY ,通过ELISA方法证实IgY抗体的特异活性。方法 :采取咽喉炎相关的多种细菌作抗原免疫产蛋母鸡 ,收集鸡蛋 ,从蛋黄中提取IgY ,经纯化后进行SDS 聚丙烯酰胺凝胶电泳和抗体活性的ELIAS测定。结果 :电泳呈现 1 2条带 ,证明IgY抗体中至少有 1 2种抗体成分 ,ELISA结果表明IgY抗体效价为1∶5 1 2。热稳定性试验结果显示 ,在 37℃温度下 ,IgY抗体活性无改变。结论 :应用多种混合的细菌抗原免疫母鸡 ,其鸡蛋黄中含有相应的IgY抗体成分 。Objective:To detect the activity of IgY against complex bacteria in pharynx and throat.Methods:Purified antigens against bacteria in pharynx and throat was used to immunize egglaid hens.The eggs from immunized hens were collected and abstract IgY from the yolks.The antibody activity of IgY was detected by SDS-PAGE electrophoresis and ELISA.Results:SDS-PAGE electrophoresis represented at least twelve ladders,and the titer of ELISA was 1∶512.Conclusion:IgY antibody was obtained in egg yolk after immunized hens with complex bacteria.The activity of IgY was detected.IgY showed stable to heat.

关 键 词：鸡蛋黄 免疫球蛋白IgY 细菌复合抗原 抗体 

分 类 号：R730[医药卫生—肿瘤]