SARS病毒S1蛋白的克隆、表达及其重组蛋白免疫学特性的鉴定  

The expression, purification and identification of recombinant SARS S1 subunit expressed in E. Coli

作　　者：李燕[1] 冯珍如[2] Sin Wan Yee 杨小昂[1] 董学员[1] 徐国宾[2] 谢雍[3] 王月丹[1] 陈慰峰[1] 

机构地区：[1]北京大学医学部免疫学系,北京100083 [2]北京大学第一医院,北京100083 [3]香港科技大学生物系

出　　处：《中国免疫学杂志》2004年第6期418-420,共3页Chinese Journal of Immunology

基　　金：教育部SARS攻关基金;973计划 (专项编号 :2 0 0 3CB5 14 10 0 );国家自然科学基金委员会 (项目批准号 ;3 0 3 40 0 11)资助

摘　　要：目的 :研究SARS病毒感染后机体产生保护性免疫应答的规律和可能机制。方法 :通过生物信息学分析 ,在原核表达系统中表达了SARS病毒S1蛋白。利用SARS流行前正常人血清和 6份SARS患者恢复期的血清 ,对纯化的重组S1蛋白进行血清学分析。结果 :研究中克隆表达的重组蛋白序列与公布的SARS病毒S1蛋白的序列相同。 6份SARS患者恢复期的血清均能识别重组S1蛋白 ,而 6份SARS流行前的正常人血清则不能与重组蛋白反应。结论 :获得的重组S1蛋白具有与天然SARS病毒S1蛋白相同的序列 ,并具有与之相似的血清学反应性 。Objective:To study the course and mechanism of the immune response to SARS virus. Methods:The recombinant SARS virus S1 subunit was expressed in E. Coli according to the results of bioinformatics analysis. After purification, the recombinant S1 protein was identified by 6 serum samples of recovered SARS patients and 6 serum samples of health donors, which were collected before out-break of SARS. Results:Sequencing analysis confirmed that the recombinant protein has the same sequence of natural SARS virus S1 subunit. The recombinant S1 protein could react with all the samples from recovered SARS patients but not the control samples from healthy donors according to the results of Western blot. Conclusion:The recombinant SARS virus S1 subunit may provide a good tool for the research of immune response to SARS virus and the producing of recombinant vaccine to prevent people from SARS.

关 键 词：SARS 冠状病毒 S蛋白 

分 类 号：R370[医药卫生—病原生物学]