Gene expression profiling related to powdery mildew resistance in wheat with the method of suppression subtractive hybridization  

作　　者：Meng Luo Xiuying Kong Naxin Huo Ronghua Zhou Jizeng Jia 

机构地区：[1]Chinese Acad Agr Sci, Inst Crop Germplasm Resources, Key Lab Crop Germplasm & Biotechnol, Minist Agr, Beijing 100081, Peoples R China

出　　处：《Chinese Science Bulletin》2002年第23期1990-1994,共5页

基　　金：This work was supported by the National "973" Program (Grant No. G1998010200) ;the National Natural Science Foundation of China (Grant No. 39980029).

摘　　要：'Bainong 3217 × Mardler' BC5F4 wheat line at the initial stage of inoculation with powdery mildew pathogen (Erysiphe graminis DC) was used to construct a suppression subtractive hybridization (SSH) cDNA library. Totally 760 ESTs were obtained through sequencing. Similarity analysis of ESTs based on BLASTn and BLASTx with the sequences in GenBank, in combination with macroarray differential screening, revealed that 199 ESTs of 65 kinds were known to be functionally disease resistance related. Based on the gene expression profiling in the present study, it is postulated that salicylic acid (SA) and MAP-related signal transduction pathways were involved in powdery mildew resistance in wheat. System acquired resistance genes were predominant in terms of kinds and quantity. With the initiation of cell defense reaction, the genes conferring anti-oxidation substances were largely expressed and thus cell protection mechanism was activated. Much evidence revealed that phenylpropanes metabolic pathway was“Bainong 3217 × Mardler” BC5F4 wheat line at the initial stage of inoculation with powdery mildew pathogen (Erysiphe graminis DC) was used to construct a suppression subtractive hybridization (SSH) cDNA library. Totally 760 ESTs were obtained through sequencing. Similarity analysis of ESTs based on BLASTn and BLASTx with the sequences in GenBank, in combination with macroarray differential screening, revealed that 199 ESTs of 65 kinds were known to be functionally disease resistance related. Based on the gene expression profiling in the present study, it is postulated that salicylic acid (SA) and MAP-related signal transduction pathways were involved in powdery mildew resistance in wheat. System acquired resistance genes were predominant in terms of kinds and quantity. With the initiation of cell defense reaction, the genes conferring anti-oxidation substances were largely expressed and thus cell protection mechanism was activated. Much evidence revealed that phenylpropanes metabolic pathway was involved in phytoalexin synthesis in wheat powdery mildew resistance. Genes conferring some enzymes of structural modification of cell walls and proteinase inhibitors inhibiting pathogen growth were also detected. The genes controlling a few proteinases (mainly cysteine proteinase) had a considerable redundancy of expression.

关 键 词：WHEAT powdery MILDEW suppression subtractive HYBRIDIZATION disease resistance-related GENES gene expression profiling. 

分 类 号：S512.103[农业科学—作物学]