Molecular identification of asymmetric somatic hybrids between wheat and Italian ryegrass  

作　　者：Taiming Ge Shunwu Yu Xinghua Lin Falan Qin Yu Yujun Yuefeng Xie 

机构地区：[1]Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China

出　　处：《Chinese Science Bulletin》2002年第24期2093-2095,共3页

基　　金：This work was supported by the Opening Project of the National Key Laboratory of Crcp Genetic Improvement.

摘　　要：Sexual incompatibility between common wheat and Italian ryegrass was an obstacle for transferring useful traits from italian ryegrass to wheat. In order to use those desirable genetic resources to improve wheat and to create new cytoplasmic germplasm, the protoplasts of wheat and Italian ryegrass were successfully electrofused and the somatic hybrid plants were regenerated. Examination with 6 restriction enzymes, 13 probes including 9 mtDNA probes (H454, Pst24, B30, Pro I, 490, B342, pHJ2-7-l, B376, 7), 3 cpDNA probes (pHvc p1, pHvc p5 and pHvc p8) and onenuclear DNA probe-pTA71 (rDNA) in total 73 enzyme/probe combinations revealed rich polymorphism between the fusion partners. RFLP analysis indicated that approximately 93.4% of the regenerated plants were true somatic hybrids. AFLP analysis implied that the somatic hybrids were highly asymmetric. The RFLP analysis using mt- and cpDNA specific probes also demonstrated the non-coexistence of mitochondria and chloroplasts from the fusion partners in theSexual incompatibility between common wheat and Italian ryegrass was an obstacle for transferring useful traits from italian ryegrass to wheat. In order to use those desirable genetic resources to improve wheat and to create new cytoplasmic germplasm, the protoplasts of wheat and Italian ryegrass were successfully electrofused and the somatic hybrid plants were regenerated. Examination with 6 restriction enzymes, 13 probes, including 9 mtDNA probes (H454, Pst24, B30, Pro I, 490, B342, pHJ2-7-1, B376, 7), 3 cpDNA probes (pHvc p1, pHvc p5 and pHvc p8) and one nuclear DNA probe—pTA71 (rDNA) in total 73 enzyme/ probe combinations revealed rich polymorphism between the fusion partners. RFLP analysis indicated that approximately 93.4% of the regenerated plants were true somatic hybrids. AFLP analysis implied that the somatic hybrids were highly asymmetric. The RFLP analysis using mt-and cpDNA specific probes also demonstrated the non-coexistence of mitochondria and chloroplasts from the fusion partners in the somatic hybrid cells.

关 键 词：WHEAT (Triticum AESTIVUM L.) Italian RYEGRASS (Lolium multiflorum Lam.) SOMATIC hybrid RFLP AFLP. 

分 类 号：S512.1[农业科学—作物学]