Extra-copy nifA enhances the nodulation efficiency of Sinorhizobium fredii  

作　　者：Zhao Jieping Dai Xiaomi Xu Ling Zhu Jiabi Shen Shanjiong Yu Guanqiao 

机构地区：[1]Laboratory of Molecular Genetics,Shanghai Institute of Plant Physiology and Ecology,Shanghai Institutes of Biological Sciences,Chinese Academy of Sciences,200032,Shanghai,China

出　　处：《Chinese Science Bulletin》2002年第7期565-567,共3页

基　　金：This work was supported by the State "863" High-Tech Programs and the fund of the Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences.

摘　　要：Previous investigations have shown that nifA gene is involved in nodulation and symbiotic nitrogen fixation regulation of Rhizobium. We study the role of nifA on nodulation of leguminous plants. We found that Sinorhizo-bium fredii harboring multi-copy plasmid carrying the con-stitutively expressed Klebsiella pneumoniae nifA exhibited an increase of nodulation activity and nodulation competitiveness on soybean plants. The Nod-factor secreted by the rhizobia cells containing the multi-copied nifA was assayed, and preliminary results showed that S. fredii containing the multi-copy plasmid carrying nifA produced higher strength of Nod-factor than the rhizobia containing the same plasmid carrying the vector did.Previous investigations have shown that nifA gene is involved in nodulation and symbiotic nitrogen fixation regulation of Rhizobium. We study the role of nifA on nodulation of leguminous plants. We found that Sinorhizobium fredii harboring multi-copy plasmid carrying the constitutively expressed Klebsiella pneumoniae nifA exhibited an increase of nodulation activity and nodulation competitiveness on soybean plants. The Nod-factor secreted by the rhizobia cells containing the multi-copied nifA was assayed, and preliminary results showed that S. fredii containing the multi-copy plasmid carrying nifA produced higher strength of Nod-factor than the rhizobia containing the same plasmid carrying the vector did.

关 键 词：SINORHIZOBIUM FREDII NIFA Nod-factor NODULATION com-petitiveness. 

分 类 号：Q945[生物学—植物学]