Isolation of osRACD gene encoding a small GTP-binding protein from rice  

作　　者：Ml Zhiyong WANG Shusheng WU Naihu 

机构地区：[1]Institute of Developmental Biology,Chinese Academy of Sciences,Beijing 100080,China [2]Academy of Life Sciences,Peking University,Beijing 100871,China

出　　处：《Chinese Science Bulletin》2002年第20期1673-1679,共7页

基　　金：This work was supported by the Fund of the State "863" and the National Basic Sciences Initiative (Grant No. 2001CB1088).

摘　　要：Using an improved version of mRNA differential display technology, we have obtained a differentially displayed fragment RDP-8. Homologous comparison indicated that the fragment RDP-8 has high homology with the gene encoding maize small GTP-binding protein. By screening cDNA library of the rice Nongken 58N panicle using the newly obtained fragment RDP-8 as probe, we further found the full-length cDNA of osRACD gene that encodes a rice small GTP-binding protein. As compared with maize RACD gene, the osRACD of rice shows remarkable homology in both nucleotide sequence and amino acid sequence, 88% and 97% respectively. Evidence from RT-PCR study indicates that osRACD gene is related to photoperiod fertility conversion of photoperiod sensitive genie male sterility (PSGMS) rice.Using an improved version of mRNA differential display technology, we have obtained a differentially displayed fragment RDP-8. Homologous comparison indicated that the fragment RDP-8 has high homology with the gene encoding maize small GTP-binding protein. By screening cDNA library of the rice Nongken 58N panicle using the newly obtained fragment RDP-8 as probe, we further found the full-length cDNA ofosRACD gene that encodes a rice small GTP-binding protein. As compared with maizeRACD gene, the osRACD of rice shows remarkable homology in both nucleotide sequence and amino acid sequence, 88% and 97% respectively. Evidence from RT-PCR study indicates thatosRACD gene is related to photoperiod fertility conversion of photoperiod sensitive genic male sterility (PSGMS) rice.

关 键 词：RICE osRACD mRNA DIFFERENTIAL DISPLAY light signal transduction. 

分 类 号：Q943[生物学—植物学]