Highly Sensitive Spectrofluorimetric Determination of Riboflavin Based on the Generation of Active Oxygen Coupled with Enzymatic Reaction  

作　　者：Li Xiao\|yan 1,2 , Wang Rui\|yong 1 , Cai Ru\|xiu 1 ,Liu zhi\|hong 1 1. College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072,Hubei, China 2. Department of Chemistry, Leshan Teacher s College, Leshan, 61 

出　　处：《Wuhan University Journal of Natural Sciences》2002年第3期361-364,共4页武汉大学学报（自然科学英文版）

基　　金：SupportedbytheNationalNatureScienceFoundationofChina (2 9775 0 15 )

摘　　要：A spectrofluorimetric method for the determining riboflavin (VB2) based on its enhancement on the fluorescence of hemoglobin-catalyzed enzymatic reaction was proposed. The proposed method consisted of two reactions. One was the photochemical reaction of VB2, the other was a hemoglobin-catalyzed enzymatic reaction. The optimal experimental conditions for the determinations were established. The linear range of the method was 5.0×10?9?1.0×10?7 mol/L of VB2. The detection limit was calculated to be 3.65×10?9 mol/L. The relative standard deviation of this method was 2.3%at 7.0×10?2 mol/L for 11 determinations.A spectrofluorimetric method for the determining riboflavin (VB2) based on its enhancement on the fluorescence of hemoglobin-catalyzed enzymatic reaction was proposed. The proposed method consisted of two reactions. One was the photochemical reaction of VB2, the other was a hemoglobin-catalyzed enzymatic reaction. The optimal experimental conditions for the determinations were established. The linear range of the method was 5.0×10?9?1.0×10?7 mol/L of VB2. The detection limit was calculated to be 3.65×10?9 mol/L. The relative standard deviation of this method was 2.3%at 7.0×10?2 mol/L for 11 determinations.

关 键 词：Key words riboflavin SPECTROFLUORIMETRIC IRRADIATION HEMOGLOBIN 

分 类 号：O657.3[理学—分析化学]