结核分枝杆菌katG基因突变的研究  

Detection of Kat G gene related with isoniazid-resistance in M. tuberculosis

作　　者：葛超荣[1] 冯立民[1] 林瑞炮[1] 钟步云[1] 陈智[1] 

机构地区：[1]浙江大学医学院附属第一医院,杭州310003

出　　处：《中华微生物学和免疫学杂志》2001年第S1期95-96,共2页Chinese Journal of Microbiology and Immunology

摘　　要：目的　了解结核分枝杆菌异烟肼耐药株KatG基因的突变情况 ,建立快速筛选耐药株的方法。方法　通过聚合酶链反应 单链构象多态性 (PCR SSCP)和聚合酶链反应 限制性片段长度多态性 (PCR RFLP)分析法对 6 4株结核菌临床分离株的katG基因的突变进行分析。结果　 46株敏感株和结核菌H37Rv标准菌株的katG基因均无SSCP泳动异常和MSPⅠ酶切位点的改变。结论　大多数结核菌耐异烟肼分离株有katG基因的突变 ,而突变位点大多是第 315位密码子AGC→ACC。PCR SS CP和PCRObjective To observe the mutations of katG gene in isoniazid-resistant M. tuberculosis strains, and to set up a rapid method for detecting the isoniazid-resistant M. tuberulosis strains. Methods Analyzing mutations of katG gene in 64 clinical isolates of M. tuberculosis by PCR-SSCP and PCR-RFLP, with H37Rv reference strain as control strain. Results No abnormalities were detected in both the SSCP and RFLP profiles of all 46 isoniazid-susceptible isolates and H37Rv strain.14 of 18(77.8%) isoniazid-resistant isolates had apparent abnormal SSCP profiles. One restricted point of MSP I was added to 10 of 14 above mentioned katG gene. No katG gene deletion was observed in all 64 isolates. Conclusions Mutation in katG gene was frequently observed in isoniazid-resistant M. tuberculosis strains and usually situated in codon 315. PCR-SSCP and PCR-RFLP might be used as a rapid, simple and sensitive method to detect isoniazid-resistance M. tuberculosis strains.

关 键 词：结核分枝杆菌 聚合酶链反应 单链构象多态性 限制性片段长度多态性 

分 类 号：R346[医药卫生—基础医学]