用单克隆抗体桥联酶标法诊断小儿呼吸道病毒感染  

Rapid diagnosis of children with respiratory tract viruses infection by monoclonal antibody bridge-linked ELISA

作　　者：孙康德[1] 王玫琦[1] 周慧君[1] 虞中敏[1] 

机构地区：[1]上海第二医科大学附属第九人民医院检验科,上海200011

出　　处：《中华微生物学和免疫学杂志》2001年第S1期114-115,共2页Chinese Journal of Microbiology and Immunology

摘　　要：目的　探讨小儿患者呼吸道病毒感染的病原诊断。方法　采用单克隆抗体桥联酶标法。结果　在 40例小儿患者中 ,阳性有 17例 ,占 42 .5 %。其中呼吸道合胞病毒 (RespiratorySyncytialVirus,RSV)占 2 0 % ,流感病毒 (InfluenzaVirus,Flu)占 12 .5 % ,副流感病毒 (ParainfluenzaVirus ,PIV)占7.5 % ,腺病毒 (Adenovirus,Adv)占 2 .5 %。结论　用单克隆抗体桥联酶标法诊断小儿呼吸道病毒是一种灵敏度高。Objective To discuss the etiology diagnosis of respiratory tract viruses infection in children in Shanghai ninth people′s hospital. Method The respiratory tract viruses were detected by monoclonal antibody bridge-linked ELISA. Results 17 samples of 40 children(42.5%) with respiratory tract infection were positive. The positive percent of Respiratory Syncytial Virus,Influenza Virus,Parainfluenza Virus,Adenovirus were 20%,12.5%,7.5%and 2.5%, respectively. Conclusion To detect respiratory tract virus infection in children, monoclonal antibody bridge-linked ELISA was a high sensitive and specific rapid method.

关 键 词：碱性磷酸酶-抗碱性磷酸酶 单克隆抗体桥联酶标技术 呼吸道病毒 

分 类 号：R446.5[医药卫生—诊断学]