脑溢安对出血性中风大鼠神经细胞凋亡的影响  

Effect of Naoyi'an on Neuronal Apoptosis in Rats after Hemorrhagic Stroke

作　　者：何泽云[1] 黎杏群[1] 何纲[1] 曾庆善[2] 贺双腾[1] 

机构地区：[1]中南大学附属湘雅医院中西医结合研究所 [2]中南大学医学院电镜室

出　　处：《中国中西医结合杂志》2001年第S1期46-48,共3页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金(No.39670907)

摘　　要：目的:探讨脑溢安颗粒剂对出血性中风大鼠神经细胞凋亡的影响。方法:用立体定位注射胶原酶方法,采用细胞形态学,DNA 裂解率及流式细胞仪技术,观察脑溢安颗粒剂对出血性中风大鼠海马 CA_1区及皮质神经细胞凋亡的影响。结果:脑出血大鼠海马 CA_1区及皮质均出现凋亡的神经细胞,脑溢安颗粒剂能减少上述区域神经细胞的凋亡。结论:脑溢安颗粒剂具有对抗出血性中风大鼠神经细胞凋亡的作用。Objective:To explore the inhibitory effects of Naoyi'an (NYA) on neuronal apoptosis in rats after intracerebral hemorrhage (ICH).Methods:Experimental ICH model rats were established by stereo-locating injection of collagenase method.Cell morphologic ex- amination,DNA fragmentation rate and flow cytometry were used to observe the effects of NYA on neuronal apoptosis of hippocampal capsula internal (CAI) region and cerebral cortex.Results:Neuronal apoptosis appeared in CAI region and cortical of ICH model rat,and it could be abated by NYA administration.Conclusion:NYA could inhibit the neuronal apoptosis in rats after ICH.

关 键 词：脑溢安颗粒剂 脑出血 神经细胞凋亡 DNA 裂解率 流式细胞术 细胞形态学 

分 类 号：R285.5[医药卫生—中药学]