芹灵颗粒对小鼠免疫性肝损伤的保护作用  

Protective Effect of Qinling Granule on Immunological Liver Injury in Mice

作　　者：杨新波[1] 黄正明[1] 曹文斌[1] 陈鸿雁[1] 张敬珍[1] 刘军 李树林[3] 

机构地区：[1]解放军北京军医学院药理教研室,北京100071 [2]解放军307医院生化检验科 [3]解放军309医院病理科

出　　处：《中国中西医结合杂志》2001年第S1期84-86,共3页Chinese Journal of Integrated Traditional and Western Medicine

摘　　要：目的:观察芹灵颗粒(QL)对卡介苗(BCG)加脂多糖(LPS)致小鼠免疫性肝损伤的保护作用。方法:选用5周龄的昆明种雄性小鼠,随机分成6组,每组8只。小鼠尾静脉注射 BCG 1 mg/只,12天后再尾静脉注射 LPS 5μg/只,造成免疫性肝损伤。造模同时给药,QL 小剂量(QLs)2.5 g.kg^(-1)组、QL 中剂量(QLm)5.0 g.kg^(-1)组、QL 大剂量(QLb)10 g·kg^(-1)组分别灌胃治疗12天,地塞米松(DEX)组在注射 LPS 30min 前腹腔注射 DEX 3 mg.kg^(-1)次,正常组和模型组小鼠分别给予 QLb 的同等剂量的生理盐水(NS)。注射 LPS6 h 后取材检测。结果:QL 能明显降低免疫性肝损伤小鼠血清谷丙转氨酶(ALT)、谷草转氨酶(AST)和谷胱甘肽 s-转移酶(GST)的水平。增加肝细胞浆中谷胱甘肽还原酶(GSH-Re)的活性,降低 MDA 的含量。在护肝同时明显抑制小鼠脾脏指数的增加。肝病理镜检证实,QLm、QLb 治疗组肝细胞水肿、变性和坏死等均比模型对照组明显减轻。结论:QL 对 BCG 加 LPS 致小鼠免疫性肝损伤有保护作用。其护肝作用与抗脂质过氧化等因素有关。Objective:To observe the protective effect of Qinling Granule (QLG) on immunologic liver injury induced by lipopolysaccharide (LPS) plus Bacillus Calmette-Guerin (BCG) in mice.Methods:Kunming male mice 5 weeks in age were randomly divided into 6 groups,8 in each group.They were,except the normal control group,modelled by injecting BCG 1 mg/mice followed by injecting LPS 5 μg/mice 12 days later through caudal vein to make immunologic liver injury.Different dosages of QLG (2,5 g/kg,5.0 g/kg and 10 g/ kg) were given to the treated groups at the same time of modelling.Dexamethasone (DEX) 3 mg/kg was given once to the positive control group 30 min before LPS injection intraperitoneaUy and for the normal control and model control group,equal dosage of normal saline was injected instead.Blood and liver of the animals were taken for observation 6 hrs after LPS injection.Results:QLG could lower the serum level of alanine transaminase,aspartate arninotransferase and glutathione-s-transferase level,increase the reduced glutathione activity and malonyldialdehyde content in hepatocytes,and inhibit the spleen index in mice significantly.Pathologic examination proved that the edema,degeneration and necrosis of hepatocytes were milder in the middle and large dosage QLG treated groups than those in the model control group significantly.Conclusion:QLG has protective effect on immunologic liver injury induced by BCG plus LPS, which might be related with anti-lipid peroxidation.

关 键 词：芹灵冲剂 免疫性肝损伤 保肝作用 

分 类 号：R285.5[医药卫生—中药学]