Light and Heat Induced Denaturation of Photosystem Ⅱ Core Antenna Complex CP47  

作　　者：王居硕 单际修 许强 公衍道 匡廷云 赵南明 

出　　处：《Tsinghua Science and Technology》1999年第3期1485-1488,共4页清华大学学报（自然科学版（英文版）

基　　金：the National Natural Science Foundation of China !(No .39890 390 )

摘　　要：Light and heat induced denaturation of CP47, the core antenna complex of photosystem Ⅱ purified from spinach, were investigated using absorption and circular dichroism spectra. Light caused the destruction of chlorophyll a and excitonic interaction of chlorophyll a in CP47, while the protein secondary structure was not apparently changed. Heat induced the destruction of protein secondary structure and excitonic interaction of chlorophyll a, but the chlorophyll a molecule was not damaged. The results suggest that both the chlorophyll a molecular structure and the protein native conformation are necessary for excitonic interaction of chlorophyll a and the energy transfer function of the chlorophyll a binding protein.Light and heat induced denaturation of CP47, the core antenna complex of photosystem Ⅱ purified from spinach, were investigated using absorption and circular dichroism spectra. Light caused the destruction of chlorophyll a and excitonic interaction of chlorophyll a in CP47, while the protein secondary structure was not apparently changed. Heat induced the destruction of protein secondary structure and excitonic interaction of chlorophyll a, but the chlorophyll a molecule was not damaged. The results suggest that both the chlorophyll a molecular structure and the protein native conformation are necessary for excitonic interaction of chlorophyll a and the energy transfer function of the chlorophyll a binding protein.

关 键 词：photosystem Ⅱ core antenna CP47 circular dichroism 

分 类 号：TN82[电子电信—信息与通信工程]