Formation of Polyhydroxyalkanoate Blends by Pseudomonas pseudoalcaligenes M1-2 from Various Carbon Sources  

作　　者：吴琼 张广 田卫东 孙素琴 张日清 陈国强 

出　　处：《Tsinghua Science and Technology》1999年第3期1539-1543,共5页清华大学学报（自然科学版（英文版）

基　　金：the Trans-Century Training Pro gramme Foundation for Talent by the Ministry of Education of China and the Major Research

摘　　要：Pseudomonas pseudoalcaligenes strain M1\|2 isolated from oil\|contaminated soil collected from an oilfield in northern China was found to be able to synthesize a blend of polyhydroxyalkanoates (PHAs) containing monomers of 3\|hydroxybutyrate (C4), 3\|hydroxyvalerate (C5), 3\|hydroxyheptanoate (C7), 3\|hydroxyoctanoate (C8), 3\|hydroxynonanoate (C9), 3\|hydroxydecanoate (C10) and 3\|hydroxydodecanoate (C12) from various carbon sources. The hydroxyalkanoate (HA) monomer composition varied both quantitatively and qualitatively, depending on the carbon sources used. The presence of octanoate in substrates of myristic acid or tridecanoate promoted the synthesis of HB monomer in the blend. Concentration of octanoate was also found to significantly affect the PHB content in the blend. A PHA biosynthesis pathway in Pseudomonas pseudoalcaligens M1\|2 was proposed. Pseudomonas pseudoalcaligenes strain M1\|2 isolated from oil\|contaminated soil collected from an oilfield in northern China was found to be able to synthesize a blend of polyhydroxyalkanoates (PHAs) containing monomers of 3\|hydroxybutyrate (C4), 3\|hydroxyvalerate (C5), 3\|hydroxyheptanoate (C7), 3\|hydroxyoctanoate (C8), 3\|hydroxynonanoate (C9), 3\|hydroxydecanoate (C10) and 3\|hydroxydodecanoate (C12) from various carbon sources. The hydroxyalkanoate (HA) monomer composition varied both quantitatively and qualitatively, depending on the carbon sources used. The presence of octanoate in substrates of myristic acid or tridecanoate promoted the synthesis of HB monomer in the blend. Concentration of octanoate was also found to significantly affect the PHB content in the blend. A PHA biosynthesis pathway in Pseudomonas pseudoalcaligens M1\|2 was proposed.

关 键 词：POLYHYDROXYALKANOATE PHA POLYHYDROXYBUTYRATE PHB Pseudomonas pseudoalcaligenes 

分 类 号：Q5[生物学—生物化学]