辅酶A口服剂对大鼠高脂血症及胰岛素抵抗的作用  

Amelioration of hyperlipidemia and insulin resistance by administration of coenzyme A oral preparation in rats

作　　者：董娜[1] 诸敏娟[2] 连榅林[1] 鲁瑾 邹大进[1] 楚正绪[2] 苏定冯[2] 陈红[2] 

机构地区：[1]第二军医大学长海医院内分泌科,上海200433 [2]药学院药理学教研室,上海200433

出　　处：《第二军医大学学报》2004年第6期655-657,共3页Academic Journal of Second Military Medical University

基　　金：上海本草生物医学工程开发基金(SMMBI20010201).

摘　　要：目的观察经保护处理的辅酶A口服剂(辅酶A加辅酶A保护剂)口服给药对大鼠食饵性高脂血症及胰岛素抵抗 的作用。方法:40只雄性SD大鼠随机分为4组,每组10只。其中1组饲以普通饲料作为正常对照组,另3组饲以高脂饮食制 备高脂血症及胰岛素抵抗模型,高脂饮食造成高血脂后每天经口分别给予辅酶A保护剂1100mg/(kg·d)、辅酶A口服剂 800或2000U/(kg·d)。治疗前后测定动物血总胆固醇、三酰甘油、低密度脂蛋白胆固醇、高密度脂蛋白胆固醇、血压及胰岛 素水平。治疗结束时同时注射葡萄糖及胰岛素后测定血糖下降速度,计算K值(代表胰岛素敏感性)。结果:给药10d后,辅 酶A口服制剂组动物总胆固醇、低密度脂蛋白胆固醇及三酰甘油水平均显著低于高脂对照组(予辅酶A保护剂)。高脂对照组 大鼠K值较正常对照组显著降低(P<0.01),而辅酶A口服制剂组[2000U/(kg·d)]大鼠K值较高脂对照组显著增高(P< 0.05)。结论:辅酶A加保护剂的口服剂可以减轻高脂饮食诱发的高脂血症及胰岛素抵抗。Objective:To evaluate the effects of an coenzyme A(CoA)oral preparation on hyperlipidemia and insulin re-sistance in rats fed on high-lipid food.Methods:Totally40male SD rats were randomly divided in to4groups(n=10).Rats in control group were fed with normal diet.Hyperlipidemia and insulin resistance rat models were established by feeding high- lipid food and rat models were further divided into CoA protective agent group [1100mg/(kg·d)]and CoA oral preparation group [800U/(kg·d)and2000U/(kg·d)].The levels of total cholesterol,triglycerides,low-density lipoprotein choles-terol,blood pressure and insulin were determined before and after treatment.Glucose and insulin were simultaneously given after treatment to determine the decreasing speed of blood glucose and the K value,which representing insulin sensitivity)was calculated.Results:Oral CoA of800to2000U/(kg·d)for10d reduced total cholesterol,low-density lipoprotein choles-terol and triglycerides dose dependently.High dose of CoA [2000U/(kg·d)]increased insulin sensitivity and high-lipid in-duced elevation in blood pressure.Conclusion:The oral preparation of CoA can ameliorate hyperlipidemia and insulin resis-tance in rats. [

关 键 词：辅酶A口服剂 大鼠 高脂血症 胰岛素抵抗 

分 类 号：R589.2[医药卫生—内分泌]