Synthesis of Novel Hydrophobic Media and Purification of Recombinant HBsAg  

作　　者：WANG Yan WANG Qun XIAO Neng-qing LIU Da-wei YE Shi-de LUO Xuan GUAN Gui-fan WEI Zi-li CHEN Wan-ge BAI Fang 

机构地区：[1]Changchun Institute of Biological Products,Changchun,130062 [2]Department of Biochemistry,Peking University,Beijing,100871 [3]Shenyang Rust Proof Packa ging Ma ter ia l Company,Shenyang,110032

出　　处：《Chemical Research in Chinese Universities》1999年第1期41-48,共8页高等学校化学研究(英文版)

摘　　要：A novel hydrophobic medium with propyl as functional group and Sepharose 6B as matrix was designed and synthesized. The comparison of the hydrophobic medium synthesized with the commercial products was made by hydrophobic interaction chromatography(HIC) in isolating recombinant hepatitis B surface antigen(r HBsAg). r HBsAg was further purified to the final products by following a downstream procedure . The results indicate that the synthesized hydrophobic medium possesses a stable structure and desired physical and chemical properties. They were used to purify r HBsAg with a high yield and purity. Both the immunity and stability of hepatitis vaccine made by the r HBsAg products have reached the same level as other similar kinds of products.

关 键 词：Chinese hamster ovary(CHO) recombinant hepatitis B surface antigen(r HBsAg) hydrophobic interaction chromatography(HIC) reverse phase haemagglutination assay(RPHA) 

分 类 号：R392.11[医药卫生—免疫学]