LPS对大鼠肺泡Ⅱ型细胞Fas抗原表达及细胞凋亡的影响(英文)  

Effect of LPS on Fas expression and apoptosis in pulmonary alveolar type Ⅱ cells

作　　者：宋勇[1] 毛宝龄[2] 夏锡荣[3] 黎介寿[1] 

机构地区：[1]南京军区南京总医院普通外科研究所,南京210002 [2]第三军医大学解放军呼吸病研究所,400037 [3]南京军区南京总医院呼吸科

出　　处：《医学研究生学报》1999年第4期229-233,共5页Journal of Medical Postgraduates

摘　　要：目的：探讨Ｆａｓ信号通路在肺泡Ⅱ型细胞损伤作用的可能机制及意义。　方法：建立大鼠肺泡Ⅱ型细胞体外培养，采用流式细胞术、原位杂交、逆转录－多聚酶链式反应等方法，观察大肠杆菌内毒素（ＬＰＳ）对肺泡Ⅱ型细胞Ｆａｓ ｍ ＲＮＡ、蛋白质及细胞凋亡的影响。　结果：成功建立大鼠肺泡Ⅱ型细胞体外培养技术，ＬＰＳ刺激后，肺泡Ⅱ型细胞Ｆａｓ ｍ ＲＮＡ、蛋白质表达均显著增加（Ｐ＜ ０．０１），ＬＰＳ可诱导肺泡Ⅱ型细胞凋亡，并在一定范围内呈量－效关系。　结论：ＬＰＳ对肺泡上皮细胞的损伤效应，除直接损伤作用外，还可能通过诱导Ｆａｓ抗原表达增加，而致细胞凋亡的方式进行。Objectives: To explore the mechanism of Fas signal pathway on damage of alveolar type Ⅱ cells. Methods: By using flow cytometry ,DNA fragmentation electrophoresis, in situ hybridization (ISH)、reverse transcription and polymerase chain reaction (RT PCR); expression of Fas mRNA and protein were studied in alveolar type Ⅱ cells in vitro. Effects of lipopolysaccharide( LPS) on apoptosis and expression of Fas were detected. Results: We successfully developed a method for isolation and culture of alveolar type Ⅱ cells in rats. Induction of apoptosis in rat alveolar type Ⅱ cell after administration of LPS; which was dependent on the dose of LPS. Conclusions: We speculate apoptosis and Fas signal pathway may play a role in LPS induced damage of alveolar type Ⅱ cells.

关 键 词：脂多糖 肺泡上皮 FAS抗原 细胞凋亡 大鼠 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]