谷子胚性悬浮细胞系植株再生体系的建立及转基因技术研究  

Establishment of Embrogenic Cell Suspension Culture and Plant Regeneration of Millet and Gene Transfer

作　　者：董云洲[1] 段胜军[2] 

机构地区：[1]中国农业科学院生物技术研究中心 [2]河北省农林科学院谷子研究所

出　　处：《应用基础与工程科学学报》1999年第1期38-44,共7页Journal of Basic Science and Engineering

摘　　要：研究建立了冀谷１１号谷子幼穗的悬浮培养细胞系及植株再生体系．用基因枪技术转化悬浮培养物，在分化培养基上获得了稳定传代的抗性愈伤组织，斑点杂交证明稳定转化愈伤组织的频率为９％～２０％．The millet cultivar, Jigu No.11, embryogenic cell suspension culture and plant regeneration directly from the cell suspension were established. Transient and stable expression of GUS gene were measured. The stable transformed cell lines with kanamycin-resistance were analysed. Dot blot demonstrated the integration of GUS gene into millet genome, and the transformation frequency reached 9%～20%.

关 键 词：谷子 幼穗 悬浮系 植株再生 基因枪 遗传转化 

分 类 号：Q78[生物学—分子生物学] Q78