大黄鱼幼鱼虹彩病毒感染的电镜研究  

Electron microscopy on iridovirus infection in Pseudosciaen crocea larva

作　　者：何爱华[1] 林奕坚 郭永建[3] 郑永和 林大鹏 

机构地区：[1]福建省卫生防疫站病毒研究室,福州350004 [2]福建省连江县水产局,连江350500 [3]福建医科大学医学检验系,福州350000

出　　处：《福建水产》1999年第3期56-59,共4页Journal of Fujian Fisheries

摘　　要：目的:探索大黄鱼幼鱼夏季高温病流行的病毒性病原体。方法:应用电子显微镜观察大黄鱼幼鱼内脏组织超薄切片。结果:发现大黄鱼幼鱼脾脏细胞肿胀,细胞内存在大量虹彩病毒颗粒。病毒颗粒直径约120nm,呈六角形,有一层蛋白质外壳,核心为核酸。病毒感染主要位于细胞质内。结论:大黄鱼幼鱼脾细胞感染的病毒是虹彩病毒,虹彩病毒感染及环境因素可能是导致大黄鱼幼鱼夏季高温病暴发流行并致死的主要原因。To investigate the pathogenic agent of summer high temperature disease of Pseudosciaena crocea larva,ultrathin sections of the spleen tissue of Pseudociaena crocea larva were observed with electuon microscopy for the evidence of ididovirus infection.Many iridovirus particles were found mainly within the cytoplasm of the spleen cells.with the morphology of hexagon with the diameter of 120nm and a protein shell.The results indicated that iridovirus could infect Pseudosciaena crocea and result in the outbreak of high temperature disease in Pseudosciaena crocea, and the viral infection in the spleen cells may be the leading cause of the fulminant death of the sick Pseudosciaena crocea larva.

关 键 词：大黄鱼(Pseudosciaena crocea) 虹彩病毒(Iridovirus) 电镜(EM.) 

分 类 号：S943[农业科学—水产养殖]