Study on Effect and Mechanism of Essential Garlic Oil in Inhibiting MonocyteVascular Endothelial Cell Adhesion Induced by Interleukin-1α  

作　　者：葛璐璐 张薇 戴云 臧燕 黄纯洁 

出　　处：《Chinese Journal of Integrative Medicine》1999年第3期170-170,共1页中国结合医学杂志（英文版）

摘　　要：Objective: To observe the effects of essential garlic oil (EGO) on vascular cell adhesive molecule 1 (VCAM 1) expression of endothelial cell and monocyte adhesion induced by interleukin 1α (IL 1α). Methods: Human umbilical vein endothelial cells (HUVEC) were isolated by pancreatin digestion method and co cultured with U937 monocyte and IL 1α or EGO+IL 1α respectively. The adhesion rate of monocyte to endothelial cell was measured and mean fluorescent intensity of immuno stained of VCAM 1 was determined by ACAS 570. Results: EGO showed significant inhibition on VCAM 1 expression induced by IL 1α either in simple culture with HUVEC or in co culture with HUVEC+U937, and could obviously down regulate the monocyte endothelial cell adhesion under effect of IL 1α. Conclusion: EGO has the effect of antagonizing against adhesion of monocyte and vascular endothelial cell, it may be due to the inhibition of EGO on adhesive molecular expression on endothelial cell.Objective: To observe the effects of essential garlic oil (EGO) on vascular cell adhesive molecule 1 (VCAM 1) expression of endothelial cell and monocyte adhesion induced by interleukin 1α (IL 1α). Methods: Human umbilical vein endothelial cells (HUVEC) were isolated by pancreatin digestion method and co cultured with U937 monocyte and IL 1α or EGO+IL 1α respectively. The adhesion rate of monocyte to endothelial cell was measured and mean fluorescent intensity of immuno stained of VCAM 1 was determined by ACAS 570. Results: EGO showed significant inhibition on VCAM 1 expression induced by IL 1α either in simple culture with HUVEC or in co culture with HUVEC+U937, and could obviously down regulate the monocyte endothelial cell adhesion under effect of IL 1α. Conclusion: EGO has the effect of antagonizing against adhesion of monocyte and vascular endothelial cell, it may be due to the inhibition of EGO on adhesive molecular expression on endothelial cell.

分 类 号：R285[医药卫生—中药学]