大鼠内侧隔核、斜角带中NOS与ChAT、NGF-R、AChE的共存神经元  

THE COLOCALIZED NEURONS OF NOS AND ChAT,NGF R AND AChE IN MEDIAL SEPTUM AND DIAGONAL BAND OF BROCA IN RAT

作　　者：金国华[1,2] 徐慧君[1,2] 武义鸣[1,2] 秦建兵[1,2] 钟世镇[1,2] 

机构地区：[1]南通医学院神经生物学研究室 [2]第一军医大学人体解剖学教研室

出　　处：《中国组织化学与细胞化学杂志》1998年第4期2-7,9,共7页Chinese Journal of Histochemistry and Cytochemistry

摘　　要：用酶组织化学和免疫组织化学双标技术，观察了正常ＳＤ大鼠基底前脑内侧隔核（ＭＳ）、斜角带垂直支（ＶＤＢ）和水平支（ＨＤＢ）中ＮＯＳ阳性神经元的形态和分布及ＮＯＳ与胆碱能神经元标志物ＣｈＡＴ、ＮＧＦ受体（ＮＧＦ－Ｒ）和ＡＣｈＥ之间的共存关系。结果发现，ＭＳ、ＶＤＢ和ＨＤＢ的头端ＮＯＳ阳性神经元较多、胞体较大、突起多，尾端ＮＯＳ阳性神经元数目较少、胞体较小、突起少而短。ＮＯＳ＋ＣｈＡＴ双标神经元占ＮＯＳ阳性神经元总数的９０％，占ＣｈＡＴ阳性神经元总数的３９％；ＮＯＳ＋ＮＧＦ－Ｒ双标神经元占ＮＯＳ阳性神经元总数的８３％，占ＮＧＦ－Ｒ阳性神经元总数的４０％；ＮＯＳ＋ＡＣｈＥ双标神经元占ＮＯＳ阳性神经元总数的９６％，占ＡＣｈＥ阳性神经元总数的３９％。这些结果为研究Ａｌｚｈｅｉｍｅｒ＇ｓｄｉｓｅａｓｅ病理过程中基底前脑隔区胆碱能神经元退变与ＮＯ的关系提供了形态学依据。Using the double labelling technique of enzyme histochemistry and immunohistochemistry, the morphology and distribution of NOS positive neurons and the colocalization of NOS and ChAT, NOS and NGF R, or NOS and AChE in neurons within the medial septum(MS), vertical and horizontal limb of diagonal band of Broca (VDB, HDB) in normal SD rat basal forebrain were studied. The results suggested that there were more NOS positive neurons with larger perikarya and more processes in the rostral region of MS,VDB and HDB, while fewer NOS positive neurons with smaller perikarya, fewer and shorter processes in the caudal region. The double labelling neurons of NOS and ChAT accounted for 90% of total NOS positive neurons and 39% of total ChAT positive neurons. The double labelling neurons of NOS and NGF R accounted for 83% of total NOS positve neurons and 40% of total NGF R positve neurons. The double labelling neurons of NOS and AChE accounted for 96% of total NOS positive neurons and 39% of total AChE positive neurons. These results provide morphological evidence for studying the relationship between degeneration of cholingergic neurons and nitric oxide within septal region in Alzheimer's disease(AD).

关 键 词：内侧隔核 斜角带 一氧化氮合酶 胆碱乙酰转移酶 神经生长因子受体 乙酰胆碱酯酶 大鼠 

分 类 号：Q26[生物学—细胞生物学]