不育症精子乳酸脱氢酶同工酶C_4的细胞化学定位、定量研究  

ENZYMIC CYTOCHEMICAL STUDIES OF LDH C 4 IN INFERTILE SPERMATOZOA

作　　者：郭玉佳[1,2] 李叔庚[1,2] 王铁霞[1,2] 高建华[1,2] 

机构地区：[1]湖南医科大学组织胚胎学教研室 [2]湖南省计划生育研究所现代优生技术重点实验室

出　　处：《中国组织化学与细胞化学杂志》1998年第4期81-87,共7页Chinese Journal of Histochemistry and Cytochemistry

基　　金：湖南省科委基金

摘　　要：本文采用酶细胞化学方法，在光镜下用积分分级计数法，全自动图像分析仪检测精子ＬＤＨ－Ｃ４活性，两法测定ＬＤＨ－Ｃ４活性显著正相关（ｒ＝０．８０１和０．７４２，Ｐ＜０．０１）；ＬＤＨ－Ｃ４酶细胞化学电镜技术观察精子ＬＤＨ－Ｃ４活性，通过计数酶点可测定ＬＤＨ－Ｃ４活性。本文检查３８例不育男性和２０例已生育男性精子ＬＤＨ－Ｃ４活性，ＬＤＨ－Ｃ４主要分布于精子ＳＴＭ及胞质，其次是顶体及质膜表面。可通过胞膜外逸到精浆，ＬＤＨ－Ｃ４在各部活性比值为５∶２∶１∶１。不育组精子ＬＤＨ－Ｃ４可能通过膜破损处外漏到精浆使精子内ＬＤＨ－Ｃ４显著减少，ＬＤＨ－Ｃ４在精子顶体亦显著减少，使不育组精子ＬＤＨ－Ｃ４活性显著低于生育组精子，提示ＬＤＨ－Ｃ４活性的定位和大小直接与生育能力相关，检测ＬＤＨ－Ｃ４活性可作为检查不育症精子质量的可靠指标。ＬＤＨ－Ｃ４与精子密度显著相关（ｒ＝０．７３７和－０．４９３，Ｐ＜０．０５）；与活动性无相关性。By means of enzymic cytochemistry, the activity of Lactate Dehydrogenase (EC 1.1.1.27) isozyme C4 (LDH C 4) in spermatozoa was determined and measured by adding up the reactive scores. At light microscopic level, the result of accounting scores was significantly positive relevant to that of image analysis ( r =0 801 and 0 742, respectively; P <0 01). At ultrastructural level, activity of LDH C 4 ws determined with enzymic ultracytochemistry. 38 infertile men and 20 fertile men were examined with these methods the results showed that LDH C 4 mainly localized in cytosols and matrix of sperm type mitochondria(STM), partly on acrosome, plasma membrane and some escaped out of membrane. The activity ratio between four areas was 5∶2∶1∶1. The LDH C 4 activity of infertile group was remarkably lower than that of fertile group ( P <0 001). It was observed that the plasma membrane of infertile spermatozoon was damaged and LDH C 4 might flow outside, thus the activity of LDH C 4 in many parts such as acrosome was greatly decreased, and the generation of LDH C 4 in spermatozoon could be relevantly lower than that in fertility. It is proved that the examination of LDH C 4 can serve as relialde item to determine spermatozoon quality. The activity of sperm LDH C 4 is clearly corelated with sperm concentration ( r =0 737 and -0 493, respectively P <0 05) but it has no corelation with sperm motility.

关 键 词：精子 不育症 LDH-C₄ 酶细胞化学 超微结构 

分 类 号：Q26[生物学—细胞生物学]