培养的海马神经元致痫后谷氨酸的合成和释放的变化  

THE CHANGES OF GLUTAMATE SYNTHETISED AND RELEASED IN CULTURED HIPPOCAMPAL NEURONS AFTER EPILEPTIC INDUCED

作　　者：徐生坤[1,2] 刘云华[1,2] 周洁萍[1,2] 朱长庚[1,2] 

机构地区：[1]同济医科大学解剖学教研室 [2]随州市第一人民医院骨科

出　　处：《中国组织化学与细胞化学杂志》1998年第4期94-98,共5页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家自然科学基金

摘　　要：通过谷氨酸（Ｇｌｕｔａｍａｔｅ，Ｇｌｕ）免疫细胞化学染色法观察到马桑内酯（ＣｏｒｉａｒｉａＬａｃｔｏｎｅ，ＣＬ）（２．５×１０－５ｍｏｌ／Ｌ）作用于体外培养的海马神经元６ｈ呈色增强，此后呈色反应明显减弱，阳性细胞数减少，胞体变小，突起短而稀少。ＭＫ－８０１（４×１０－５ｍｏｌ／Ｌ）可降低ＣＬ引起的海马神经元Ｇｌｕ免疫反应性。高效液相色谱法（ＨＰＬＣ）测定ＣＬ作用于培养的海马神经元２４ｈ后培养基中Ｇｌｕ和天门冬氨酸（Ａｓｐ）含量增加（Ｐ＜０．００１），ＭＫ－８０１并不能阻断此种效应。结果提示ＣＬ致痫后，早期神经元内Ｇｌｕ合成增加，后期向胞外释放。The hippocampal neurons in culture wre immunocytochemically stained with anti glutamate serum. The positive neurons stained strongly 6h after CL administration (2 5×10 -5 mol/L). The number of positive neurons decreased with smaller cell bodies and shorter, thicker processes. MK 801(4×10 -5 mol/L) partly weakened the CL induced Glu immunoreactivity of hippocampal neurons. With reversed phase high performance liquid chromatography (HPLC), glutamate and aspartate of culture media significantly increased after hippocampal neurons had been challenged with CL for 24h ( P <0 001). MK 801 did not block the above changes. These results suggest that the epileptic hippocampal neurons induced by CL produce more glutamae in earlier stage, and later release it to outside of neurons.

关 键 词：海马 细胞培养 谷氨酸 癫痫 

分 类 号：Q26[生物学—细胞生物学]