人类胃组织复合表达两种不同剪接的胆囊收缩素-B/胃泌素受体  

Human Gastric Tissues Coexpress Two Different Splicing Cholecystokinin-B /Gastrin Receptors

作　　者：周建奖[1] 胡建昆[2] 陈志新[2] 王文玲[3] 张群洲 陈曼玲[1] 

机构地区：[1]四川大学基础医学院生化与分子生物学教研室,成都610041 [2]四川大学华西医院外科,成都610041 [3]贵阳医学院附属医院肿瘤科,贵阳550004 [4]Oral & Maxillofacial Department, Charles R. Drew University of Medicine and Science

出　　处：《生物医学工程学杂志》2004年第3期440-443,共4页Journal of Biomedical Engineering

摘　　要：为了探讨正常和异位剪接的胆囊收缩素 - B/胃泌素受体基因是否在人类胃上皮细胞中表达及其表达量与胃癌分化和转移的关系。检测了 30例胃癌和癌周正常胃组织上皮细胞、10例胃炎和 2例胃正常组织上皮细胞及胃癌细胞株 SGC- 790 1中这两种受体基因的表达水平。结果显示 ,正常、炎症和恶性胃上皮细胞同时表达正常和异位剪接的胆囊收缩素 - B/胃泌素受体 ,两种受体的表达量与胃癌的分化与转移无关。推测异位剪接的胆囊收缩素This study was conducted to explore whether cholecystokinin-B/gastrin receptor (CCKBRwt) gene and its alternative splicing variant (CCKBRi4sv) are expressed in human gastric carcinomas cell line and tissues, and to find out their relationship with the development and progression of human gastric carcinoma. The mRNA expression levels of CCKBRwt and CCKBRi4sv were detected in 30 human gastric carcinomas and normal tissues adjacent to cancer, 10 gastritis specimens, 2 autopsied normal stomach specimens as well as in a gastric carcinoma cell line SGC-7901 cells. The results revealed that the transcripts of CCKBRwt and CCKBRi4sv were observed in all of the human gastric specimens tested, but only CCKBRwt was expressed in gastric cancer cell line SGC-7901 cells. The expression levels of the two receptors were not correlated with the differentiation and metastases of gastric cancers. From the results, we infer that human gastric tissues simultaneously express CCKBRwt and CCKBRi4sv, and CCKBRi4sv may have unknown physiological functions in gastric epithelial cells.

关 键 词：胆囊收缩素-B 胃泌素受体 选择性剪接 胃上皮细胞 胃癌细胞株 

分 类 号：R341[医药卫生—基础医学]