Identification and characterization of inward K^+ -channels in plasma membranes of Arabidopsis root cortex cells  

作　　者：于川江 武维华 

出　　处：《Science China(Life Sciences)》1999年第3期307-315,共9页中国科学（生命科学英文版）

基　　金：Project supported by the National Natural Science Foundation of China (Grant No. 39470359)

摘　　要：Patch clamping whole-cell recording techniques were applied to study the inward K + -channels in ,Ara-bidopsis root cortex cells. The inward K+-channels in the plasma membranes of the root cortex cell protoplasts were activated by hyperpolarized membrane potentials. The channels were miglv selective tor K + ions over Na + ions. The channel activity was significantly inhibited by the external TEA+, or Ba2+ The changes in eytoplasmic Ca2+ concentrations did not affect the whole-cell inward K + -currents. The possible asoeiation between the ehannel selectivity to K+ and Na+ ions and plant salt-toleranee was also discussed.Patch clamping whole-cell reeording techniques were apphed to study the inward K⁺ channels in Arabidopsis root cortex cells. The inward K⁺-channels in the plasma membranes of the root cortex cell protoplasts were activated by hyperpolarized membrane potentials. The channels were highly selective tor K⁺ ions over Na⁺ ions. The channel activity was significantly inbibited by the external TEA（?） or Ba（?） The changes in cytoplasmic Ca²⁺ concentrations did not affect the whole-cell inward K⁺-currents. The possible asso（?）ation betw（?）en the channel selectivity to K⁺ and Na（?） ions and plant salt-tolerance was also discussed.

关 键 词：Arabidopsis root CELLS K+-channels K+/Na+ selectivity. 

分 类 号：Q942[生物学—植物学]