Enzyme-catalyzed reaction of voltammetric enzyme-linked immunoassay system based on OAP as substrate  

作　　者：张书圣 陈洪渊 焦奎 

出　　处：《Science China Chemistry》1999年第2期195-203,共9页中国科学（化学英文版）

基　　金：Project supported by the National Natural Science Foundation of China (Grant No. 29775012)

摘　　要：Theo-aminophenol (OAP)-H2O2-horseradish peroxidase (HRP) voltammetric enzyme-linked immunoassay new system has extremely high sensitivity. HRP can be measured with a detection limit of 6.0×1010 g/L and a linear range of 1.0×10-9-4.0×10-6 g/L. The pure product of H2O2 oxidizing OAP catalyzed by HRP was prepared with chemical method. The enzyme-catalyzed reaction has been investigated with electroanalytical chemistry, UV/Vis spectrum, IR spectrum,13C NMR,1H NMR, mass spectrum, elemental analysis, etc. Under the selected enzyme-catalyzed reaction conditions, the oxidation product of OAP with H2O2 catalyzed by HRP is 2-aminophenoxazine-3-one. The processes of the enzyme-catalyzed reaction and the electroreduction of the product of the enzymecatalyzed reaction have been described.The o-aminophenol （OAP）-H₂O₂-horseradish peroxidase （HRP） voltammetric enzyme-linked immunoassay new system has extremely high sensitivity. HRP can be measured with a detection limit of 6.0×10^-（10） g/L and a linear range of 1.0×10^-9—4.0×10^-6 g/L. The pure product of H₂O₂ oxidizing OAP catalyzed by HRP was prepared with chemical method. The enzyme-catalyzed reaction has been investigated with electroanalytical chemistry, UV/Vis spectrum, IR spectrum, ¹³C NMR, ¹H NMR, mass spectrum, elemental analysis, etc. Under the selected enzyme-catalyzed reaction conditions, the oxidation product of OAP with H₂_O2 catalyzed by HRP is 2-aminophe-noxazine-3-one. The processes of the enzyme-catalyzed reaction and the electroreduction of the product of the enzymecatalyzed reaction have been described.

关 键 词：O-AMINOPHENOL HORSERADISH PEROXIDASE VOLTAMMETRY enzyme-catalysis electrochemical immunoassay. 

分 类 号：O643.3[理学—物理化学]