红景天对阿尔茨海默病APP-C100转基因小鼠的影响  

Effects of Rhodiola on Alzheimer pathology and open field activity in APP-C100 transgenic mice

作　　者：朱爱琴 [1] Qiao-xin Li  张鑫生 [1] 滕长青 [1] 楮以德 [1] Colin L Masters  Amee George  Tian Cardamong  Genevieve Evin  

机构地区：[1]青海省人民医院老年病研究所,青海,西宁,810007 [2]Department of Pathology,University of Melbourne,Australia

出　　处：《中国老年学杂志》2004年第6期530-533,共4页Chinese Journal of Gerontology

基　　金：中国教育部国家留学基金资助项目〔留金出 (2 0 0 2 ) - 2 31〕

摘　　要：目的　探讨红景天对 AD转基因小鼠行为的影响和抗氧化应激反应的神经保护作用。方法　用 APP- C1 0 0转基因小鼠和健康(NTg)鼠模型来观察单剂红景天 8w抗氧化效果。Open field活动用来评估转基因鼠和健康鼠的移动、探索能力及焦虑状态。 Western印迹法定量分析转基因鼠血清及大脑 Cn/ Zn SOD1、APP及 Aβ蛋白含量。免疫组织化学法观察转基因鼠大脑皮层和海马的病理改变。结果　与 NTg鼠比较 ,转基因鼠通过格子次数 (grid cross)和排泄物 (粪尿 )均显著增加 (P<0 .0 5)。通过 Western印迹法及密度定量测定 ,红景天治疗组的转基因鼠 Cn/ ZnSOD1蛋白含量在血清及大脑中较对照组明显减少 (P<0 .0 5)。大脑 APP、βCTF及 Aβ蛋白含量无变化 ,免疫组化在大脑皮层和海马未见 Aβ沉积。结论　红景天可通过减少 SOD1氧化蛋白的含量 。Objective To investigate the effect of Rhodiola on Alzheimer pathology and open field activity of transgenic mice Methods The transgenic APP C100 mice and healthy mice (NTg) models were used to observe the antioxygenic property of Rhodiola Open field activity was used to evaluate the ability of move and search and the situation of anxiety of transgenic mice and healthy mice The contents of Cu/ZnSOD1, APP, β CTF and Aβ in serum and brain of transgenic mice were measured by Western blot, and the pathology changes of cerebral cortex and hippocampi of transgenic mice were observed by immunohistochemistry Results The open field activities of the transgenic mice were increased compared with the Tg mice significantly ( P <0 05), and the open field activities of the transgenic mice with Rhodiola treatment were decreased compared with controls The contents of Cn/ZnSOD1 in the serum and brain of the transgenic mice with Rhodiola were significantly lower than those of control group ( P <0 05) There were no significant of APP, β CTF and Aβ level in brain between treatment group and control group Furthermore, amyloid plaques were not observed in brain cortex and hippocampi of two transgenic mice groups Conclusions Rhodiola can affect the ability of move and search and the situation of anxiety and improve the behavior of transgenic mice Rhodiola can inhibit the oxidative stress reaction in serum and brain of transgenic mice by decreasing the contents of SOD1

关 键 词：阿尔茨海默病 氧化应激 活动 抗氧化剂 转APP-C100基因鼠 

分 类 号：R967[医药卫生—药理学]