酶联免疫吸附法检测肌酸激酶B亚基含量  

MEASURING THE AMOUTNT OF B SUBUNIT IN CREATINE KINASE WITH EL ISA METHOD

作　　者：万清芝 徐淑美[1] 栾树礼 曹岩[2] 陈培勋[2] 

机构地区：[1]青岛市第二人民医院内科,青岛266033 [2]青岛医学院生物化学教研室

出　　处：《齐鲁医学杂志》1998年第4期245-247,共3页Medical Journal of Qilu

基　　金：山东省教委科研基金资助课题

摘　　要：1目的　建立酶联免疫吸附法 (EL ISA)检测肌酸激酶 B亚基含量。 2方法　应用脑肌酸激酶抗原制备单克隆抗体 ,采用对酶活力具有激活或抑制的单克隆抗体配对建立 EL ISA法 ,并应用其检测肌酸激酶 B亚基含量。 3结果　肌酸激酶 B亚基含量在 0 .0 5～ 5 0 .0 0 ng间与 A45 0 nm呈半对数直线关系。 4结论　采用建立的 EL ISA法可测定肌酸激酶Objective To establish an enzyme link imm unosorbent assay(ELISA) for measuring the amount of B subunit of creatine kinase(CK) . Methods The monoclonal antibodies were prepared using the cerebral CK as an antigen.The ELISA method could be established by matching the activating and inhibiting monoclonal antibodies.The amountof B subunitof CK could be measured by the EL ISA method. Results In the range of0 .0 5— 5 0 .0 0 ng,the am ount of B subunit of CK and A45 0 nm of EL ISA were sem ilog-straight line type. Conclusion The established ELISA method m ight measure the am ount of B subunit of CK.

关 键 词：肌酸激酶 酶联免疫吸附法 抗体 单克隆 

分 类 号：R445.6[医药卫生—影像医学与核医学]