酶标记羊抗兔间接法检测烟草马铃薯Y病毒的研究  

Studies on Tobacco PVY Delection Using Indirect Elisa

作　　者：郭兴启[1] 朱汉城[1] 吕士恩[1] 王智发[1] 陈瑞泰 

机构地区：[1]山东农业大学植保系,泰安271018

出　　处：《中国烟草学报》1998年第2期38-42,共5页Acta Tabacaria Sinica

基　　金：中国烟草总公司资助

摘　　要：本文是对辣根过氧化物酶标记羊抗兔IgG间接法检测浸染烟草的马铃薯Y病毒（PVY）的报导。试验证明该方法检测烟草植株内的PVY具有特异性，健株对照及非PVY病毒均为阴性，检测烟草植株汁液的稀释度可达1／1000，在我们的测试条件下，第一抗血清浓度1／2000，测试抗血清（酶标记羊抗兔）浓度1／140为好。The present paper deals with the study of detecting potato virus Y (PVY) intobacco plant using indirect ELISA, HRP-Antirabit goat globulin has been peed in theexperiment .The result showed that the difference between PVY average OD492 Value and CKaverage OD492 Value is very obvious, the ratio of PVY average OD492 Value to CK average OD492 Value was more than 2. Dilution of PVY in tobacco Plant, which could bedetected, was 1/1000. The method is effective and specific for detpeting PVY iu tobaccoplant.

关 键 词：马铃薯Y病毒 酶联免疫吸附试验 酶标记羊抗兔IgG 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]