异种血清诱发肝纤维化模型中ECM变化的研究  

CHANGES OF EXTRACELLULAR MATRIX IN THE HETEROSERUM-INDUCED RAT LAVER FIBROSIS MODEL

作　　者：黄志刚[1] 翟为溶[1] 张月娥[1] 张秀荣[1] 

机构地区：[1]上海医科大学基础医学院病理学教研室,上海200032

出　　处：《中国组织化学与细胞化学杂志》1998年第2期135-138,246,共5页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家自然科学基金;上海市教委重点学科(病理学)资助!39330140

摘　　要：为了观察异种血清诱发肝纤维化过程中细胞外基质（ECM）及其生成细胞的变化规律，经腹腔注射猪血清建立大鼠肝纤维化模型，以免疫组织化学方法显示肝内胶原（Col）Ⅰ、Ⅲ、Ⅳ、Ⅴ型和纤连蛋白（Fn）及其生成细胞。结果：①Fn最早在汇管区、小叶间隔内沉积，ColⅢ、Ⅳ、Ⅴ随之增多，停止血清注射后它们都明显减弱或消失；ColⅠ沉积出现最迟，但阳性迅速增强，停止注射后仍较强；②结蛋白（Dm）阳性的肝星状细胞（HSC，原称Ito细胞）其分布和数量变化与Fn和ColⅡ基本同步，而位于汇管区及细胞间隔的原始间叶细胞（PMC）Dm（-）。提示ECM生成细胞可能来自细胞骨架表型不同的PMC和HSC，本模型由于能清晰地显示ECM及其生成细胞而更适用于肝纤维化机制及防治的研究。In order to obeserve the changes of the deposition of extracellular matrix (ECM) and the ECM-producing cells during the heteroserum-induced liver fibrogenesis, rat liver fibrotic model was established by injecting intraperitoneally with porcine serum. Forty wistar female rats, weighing about 100 g, were injected the serum 0. 5 ml twice a week for 10 weeks, liver fibrosis developed since the end of the 8th week. Collagen (Col) type Ⅰ, Ⅱ, Ⅳ, Ⅴ, fibronectin (Fn) and desmin (Din) were visualized by immunohistochemical method. The results showed that the deposition of Fn was the earliest event followed by that of Col Ⅲ, Ⅳ,Ⅴ in the portal tract and the sepia, and diminished remarkably after the end of the injection,while the deposition of Col I appeared the latest but it increased rapidly and maintained at high level even after discontinuance of injection. Changes of the distrubution and number of hepatic stellate cells marked by Din were basically synchronized with that of Fn and Col Ⅲ.while the primary mesenchymal cells within the portal tract and cellular sepia were Dm negative. This modal clearly presented the dynamic changes of ECMs and their relation to the producing cells, therefore it might be more applicable for studying the mechanism of liver fibrogenesis.

关 键 词：异种血清 ECM 肝纤维化 大鼠 

分 类 号：S852.4[农业科学—基础兽医学]