大部分肝切除后肝再生中TNFα、IL6作用的研究  

A STUDY OF TUMOR NECROSIS FACTOR α (TNF α) AND INTERLEUKIN 6 (IL 6) IN THE LIVER REGENERATION AFTER PARTIAL HEPATECTOMY

作　　者：唐望先[1,2] 杜荔菁[1,2] 张文英 陈春莲[1,2] 

机构地区：[1]同济医科大学肝病研究所 [2]同济医院病理科

出　　处：《中国组织化学与细胞化学杂志》1998年第3期69-72,共4页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家教委回国人员基金

摘　　要：为研究ＴＮＦα、ＩＬ６在肝再生中的作用，我们制备了大鼠７０％肝切除后肝再生的模型，以增殖细胞核抗原（ＰＣＮＡ）的免疫组织化学染色作为肝再生的指标，对其ＴＮＦα、ＩＬ６的水平进行了检测。结果显示：ＰＣＮＡ在肝切除后残存肝组织中的表达明显升高，于手术后２４小时达高峰。手术后３小时，假手术组和肝切除组血中ＴＮＦα和ＩＬ６的水平均有升高，但假手术组很快恢复至正常，而肝切除组血清ＴＮＦα和ＩＬ６的水平继续升高，并于术后１２小时达高峰（Ｐ＜００５）。研究提示：ＴＮＦα。In order to determine the effect of TNF α and IL-6 in liver regeneration after partial hepatectomy, the model was established by partial hepatectomy. Liver regeneration was assessed by immunohistochemical staining of proliferating cell nuclear antigen (PCNA). Serum TNF α and IL 6 levels were measured with ELISA. The results showed that the expression of PCNA was increased in remnant liver tissues and reached the highest beak 24 hours after partial hepatectomy. The percentage of positive cells was as high as 54%. The levels of TNF α and IL 6 were higher in groups of PH than those in groups of sham operation. The levels of TNF α and IL 6 increased gradually and reached a maximum level at 12 hour after PH. We conclude that PCNA is an important index of cell regeneration and TNF α and IL 6 play an importent role in cell proliferation.

关 键 词：部分肝切除 肝再生 增殖细胞核抗原 肿瘤坏死因子 白细胞介素6 

分 类 号：Q593.1[生物学—生物化学]