用原位末端标记方法观察细胞凋亡在正常喉粘膜、炎症、癌前和癌变中的作用  

DETECTION OF APOPTOSIS IN NORMAL, INFLAMMATORY, PREMALIGNANT AND MALIGNANT LARYNGEAL MUCOSA BY IN SITU END LABELING METHOD

作　　者：邓志宏[1] 王剑波[1] 金岩[1] 黄维国[1] 邱建华[1] 

机构地区：[1]第四军医大学西京医院耳鼻喉科

出　　处：《中国组织化学与细胞化学杂志》1998年第3期96-99,共4页Chinese Journal of Histochemistry and Cytochemistry

摘　　要：为了观察凋亡与喉粘膜癌变的关系，本实验应用原位末端标记（ＩＳＥＬ）方法检测喉粘膜正常（ｎｏｒｍａｌｌａｒｙｎｇｅａｌｍｕｃｏｓａ，Ｎ）、炎症（ｉｎｆｌａｍｍａｔｉｏｎ，ＩＦ）、不典型增生（ｄｙｓｐｌａｓｉａ，ＤＹＳ）及鳞癌（ｓｑｕａｍｏｕｓｃｅｌｃａｒｃｉｎｏｍａ，ＳＣＣ）中细胞凋亡情况。标记后的凋亡细胞通过计数以凋亡指数（ＡＩ）表示。结果表明：ＤＹＳ组的凋亡指数（ＡＩ）在所有病变中最高；ＤＹＳ及ＳＣＣ组的ＡＩ值较正常Ｎ及ＩＦ组明显增多，且有显著性差异（Ｐ＜００５）；对ＳＣＣ的计数显示，ＡＩ值随着ＳＣＣ的分化程度下降有逐渐减少的趋势。ＳＣＣ转移组与非转移组的ＡＩ值间无显著性差异（Ｐ＞００５）。结论：细胞凋亡在喉粘膜癌前病变和癌变中发挥着重要作用。To evaluate the relationship between apoptosis and carcinogenesis of laryngeal mucosa, the distributions of apoptosis in normal (N), chronic inflammation (IF), dysplasia (DYS) and squamous cell carcinoma (SCC) of laryngeal mucosa were analyzed by in situ end labeling method. The apoptosis index (AI) represented the percentage of positive labeled apoptotic cells in all measured cells. The results showed that AI of DYS was the highest one in all observed groups. The AIs of DYS and SCC were significantly higher than N and IF ( P <0 05). The AI of well differentiated SCC was higher than poorly differentiated SCC. Our results indicate that apoptosis plays an important role in premalignant lesion and malignancy of laryngeal mucosa.

关 键 词：原位末端标记 喉粘膜 癌前病变 鳞癌 

分 类 号：Q26[生物学—细胞生物学] R767[医药卫生—耳鼻咽喉科]