地塞米松和肺成纤维细胞对大鼠胎肺表面活性物质合成的影响  

THE EFFECTS OF DEXAMETHASONE AND LUNG FIBROBLASTS ON SYNTHESIS OF FETAL RAT PULMONARY SURFACTANT

作　　者：徐锦[1] 王晓川[1] 金勤立[1] 夏绿蒂[1] 

机构地区：[1]上海医科大学儿科医院儿科研究所

出　　处：《中国组织化学与细胞化学杂志》1998年第3期110-115,共6页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家教委博士点基金

摘　　要：为提高ＤＥＸ对新生儿呼吸窘迫综合征（ＮＲＤＳ）的防治，本文研究了地塞米松（ＤＥＸ）促进肺表面活性物质（ＰＳ）合成的作用机制。用分离培养的２０天大鼠胚胎肺泡Ⅱ型细胞为材料，观察了单独用ＤＥＸ、用与不用ＤＥＸ刺激后的成纤维细胞条件培养液（ＤＥＸＦＣＭ、ＦＣＭ）对肺泡Ⅱ型细胞ＰＳ中磷脂合成及特异性肺表面活性物质蛋白质ＳＰＢ、ＳＰＣｍＲＮＡ表达的影响。结果表明，ＤＥＸ本身及未用ＤＥＸ刺激的ＦＣＭ对肺泡Ⅱ型细胞的上述三种指标均没有影响，而ＤＥＸ刺激后的ＤＥＸＦＣＭ使这三个指标有不同程度的增加效应。In order to study the effect of Dexamethasone (DEX) on synthesis of pulmonary surfactant (PS), the fetal rat primary alveolar type Ⅱ cells were purified and cultured. The influence of DEX and lung fibroblasts on the alveolar type Ⅱ cells were observed. By measuring the incorporation of 3H-choline into phosphotidycholine(PC) and using the method of RT PCR, the synthesis of PC and the expression of SP B、SP C mRNA of alveolar type Ⅱ cells were examined. These results showed that DEX alone or the fibroblast conditioned medium (FCM) untreated with DEX has no effect on the incorporation rate of 3H choline into PC and the expression of SP B、SP C mRNA of alveolar type Ⅱ cells, whereas these three indexes demonstrate distinct stimulation after being cultured with DEX FCM. It suggests that fibroblasts may be the medium of glucocorticoids action on alveolar type Ⅱ cells.

关 键 词：地塞米松 肺泡Ⅱ型细胞 成纤维细胞 肺表面活性物质蛋白质 逆转录PCR 细胞培养 

分 类 号：Q955[生物学—动物学] Q26