溃疡病胃窦幽门腺β-内啡肽样免疫反应细胞体视学分析  

STEREOLOGICAL ANALYSIS OF THE β ENDOPHIN LIKE IMMUNOREACTIVITY CELLS IN ANTRAL PYLORIC GLANDS OF PATIENTS WITH PEPTIC ULCER

作　　者：高增贺[1,2] 周济远[1,2] 任君旭[1,2] 白雪梅 郑慧娥[1,2] 张耕 徐根兴[1,2] 

机构地区：[1]张家口医学院外科学教研室 [2]南京大学生物教研室

出　　处：《中国组织化学与细胞化学杂志》1998年第1期110-114,共5页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家自然科学基金

摘　　要：应用真彩色图象分析仪，对１５例ＤＵ、ＧＵ和Ｃｏｎ组患者，非病变区胃窦粘膜幽门腺，１５１个β－内啡肽样免疫反应细胞（β－ＥＰ细胞）的１２个参数进行检测及每组７５个单位面积（５０００μｍ２）的β－ＥＰ细胞进行计数。β－ＥＰ细胞的周长、面积、长径、短径和平均体积数值是ＤＵ组＜ＧＵ组＜Ｃｏｎ组，各组间两两相比，均呈高度显著性差异，Ｐ＜０．０１。在ＤＵ与Ｃｏｎ组、或ＧＵ与Ｃｏｎ组之间，比表面、平均截距、平均表面积和平均直径数值相比，亦呈高度显著性差异，Ｐ＜０．０１。ＤＵ组β－ＥＰ细胞数增高，与ＧＵ或Ｃｏｎ组分别相比，均呈高度显著性差异，Ｐ＜０．０１。在三组中，β－ＥＰ细胞的比表面积、平均截距、平均表面积、平均直径数值和细胞数的显著性差异率在６０％以上，周长、面积、长径、短径和平均体积数值达１００％。本文首次建立了ＤＵ、ＧＵ和Ｃｏｎ组非病变区胃窦粘膜幽门腺β－内啡肽细胞的二维和三维形态计量学参数数据，提示β－ＥＰ细胞参与了溃疡病的内分泌调控活动。With color image analyser, 12 parameters on 151 β endorphin immunoreactivity cells (β EP cells) and the number of β EP cells on 75 unit area (5000μm 2) were measured in pyloric glands of the unaffected part of antral mucosa from 15 cases including the duodenal ulcer (DU) and gastric ulcer(GU) and superficial gastritis as control group (Con). The data of boundary (B), area (A), long diameter (LD), short diameter (SD) and mean volume (V) of β EP cells were lower in DU group than in GU, but the data were lower in GU group than in Con group. A highly significant difference was found between them, respectively (p<0 01). The number of the specific surface (δ), mean intercept(), mean surface () and mean diameter (c) of β EP cells also revealed highly significant difference between DU and Con or between GU and Con groups, p<0 01. The number of β EP cells (N) was increased and showed hightly significant difference in DU group as compared with GU or Con groups, p<0 01. The sigificant differential rate of δ,, AKD-]c and N was above 60% and that of B, A, LD, SD and V was 100% among three groups, respectively. A data base of 2 and 3 dimensional morphometeric parameters of β EP cells in DU, GU and Con groups was first established. These findings suggested that β EP cells participated in endocrine regulation during peptic ulcer.

关 键 词：β-内啡肽细胞 体视学 幽门腺 溃疡病 

分 类 号：R392.12[医药卫生—免疫学] R573.1[医药卫生—基础医学]