小柴胡汤水溶性物质对接受PHA刺激的PBMC产生IL-2以及细胞CD抗原变化的研究  

THE EFFECTS OF WATER SOLUBLE SUBSTANCE EXTRACTED FROM XIAO CHAI-HU-TANG ON IL-2 PRODUCTION AND CD ANTIGEN CHANGE OF PHA-STIMULATED PBMC

作　　者：刘佳佳[1] 薛华[1] 陈正芳[1] 

机构地区：[1]泸州医学院微生物学和免疫学教研室,646000

出　　处：《泸州医学院学报》1997年第5期331-334,共4页Journal of Luzhou Medical College

基　　金：国家中医药管理局青年科研基金

摘　　要：运用逐级过滤、离心和真空干燥技术,从小柴胡汤水煎液中提取到完全溶解于水的物质。经酶联免疫吸附试验(ELISA)测定.这种水溶物在体外同植物血凝索(PHA,5μg/ml)协作作用于外周血单个核细胞(PBMC).具有依浓度梯度促进白细胞介素2(IL-2)产生的作用,并且瓶底的培养细胞经 CD单抗 S-P一步法测定,CD_4阳性 T 淋巴细胞明显增多,这种变化也同小柴胡汤水溶物的浓度呈正相关。实验结果提示:小柴胡汤在临床上对某些难治性疾病的治疗效果,可能同其水溶物中某些成份参与 IL-2这一免疫应答和免疫调控的中心环节有关。By means of stepwise filtration,centrifugation and vacuum drying technique,water soluble substance was extracted from Chinese compound medicine-Xiao Chai-hu-tang.The water sol- uble substance enhanced the IL-2.These effects were concentration gradient dependent.The data suggest that Xiao Chai-hu-tang plays an important role in immune response and immunomodulation, which might be an important part of the treatment mechanism of Xiao Chai-hu-tang to autoimmune diseases.

关 键 词：小柴胡汤 水溶物 白细胞介素2(IL-2) 分化群抗原(CD) 

分 类 号：R285.5[医药卫生—中药学]