正交设计在确定混合血清法检测丙型肝炎抗体实验条件中的应用  

APPLICATION OF ORTHOGONAL DESIGN TO ESTABLISH THE EXPERIMENTAL CONDITIONS FOR DETECTING THE ANTIBODY TO HCV IN POOLED SERA

作　　者：刘沛 史志旭 张新英 张言超 

机构地区：[1]徐州市中心血站 [2]传染病学教研室

出　　处：《徐州医学院学报》1997年第6期17-19,共3页Acta Academiae Medicinae Xuzhou

基　　金：江苏省卫生厅资助

摘　　要：目的探讨封闭温度、时间、ＢＳＡ浓度、Ｔｗｅｎ２０浓度及混合标本个数５因素各２水平对混合血清法检测抗－ＨＣＶ的影响。方法应用正交设计的方法进行实验分组，采用ＥＩＡ法检测抗－ＨＣＶ。结果封闭条件为０．５％ＢＳＡ，３７℃，３０ｍｉｎ；洗涤液为０．１５％Ｔｗｅｅｎ２０，混合标本个数为５。采用正交设计的实验方法可减少７５％的实验次数。结论在进行多因素的实验研究时，正交设计是一种高效的设计方法。Objective To establish the conditions for detecting the antibody to HCV in pooled sera.Methods 5 factors at either of their 2 levels were arranged according to an orthogonal design.The antibody to HCV in pooled sera was tested by using EIA.Results The optimized conditions were blocking with 0.5% BSA at 37℃ for 30min,washing with 0.15% Tween 20 and mixing 5 serum samples as one pool.Ordinarily,25 or 32 tests are required to obtain results similar to these, implying that 75% of labor and materials can be saved by adopting the orthogonal design.Conclusion Orthogonal design is worthy of adoption for high-efficiency multifactorial experimentation.

关 键 词：正交设计 混合血清法 抗-HCV 

分 类 号：R181.36[医药卫生—流行病学]