骤冷与饥饿对小鼠肝脏影响的实验研究  

EXPERIMENTAL STUDY OF THE EFFECT OF STARVING AND ACUTE COLD ON MICE LIVER

作　　者：许晓利 张艳[1,2] 熊希凯[1,2] 

机构地区：[1]武汉市职工医学院解剖学教研室 [2]同济医科大学解剖学教研室

出　　处：《中国组织化学与细胞化学杂志》1997年第3期54-59,共6页Chinese Journal of Histochemistry and Cytochemistry

摘　　要：为探讨饥饿及饥饿与骤冷对动物肝脏的影响，本实验用健康昆明种小鼠２５只，随机分成正常组５只，饥饿组１０只，饥饿后再予冷刺激组１０只（下称骤冷组）。采用组织化学及酶组织化学方法观察糖原（ＰＡＳ反应）、ＳＤＨ（琥珀酸脱氢酶），ＬＤＨ（乳酸脱氢酶），ＣｈＥ（胆碱酯酶）、Ｍｇ２＋－ＡＴＰａｓｅ（镁激活三磷酸腺苷酶），ＡＣＰ（酸性磷酸酶）。结果提示：饥饿时肝脏ＰＡＳ反应，ＳＤＨ，Ｍｇ２＋－ＡＴＰａｓｅ、ＣｈＥ活性显著下降，而ＡＣＰ活性明显增强；饥饿后骤冷时ＰＡＳ（反应）、ＳＤＨ、ＣｈＥ更显著下降，而ＡＣＰ及Ｍｇ２＋－ＡＴＰａｓｅ活性反而增强。In order to search for the effect of starving and exerted acute cold on animal lives, histochemical and enzymehistochemical methods were used 25 on mice of Kun-Ming species, weighing 25-35 g, were diveded at random into three groups. 5 for normal group, ten for starving group another ten for starving and exerted acute cold group. The reaction of PAS, SDH, LDH, ChE, Mg 2+ ATPase, ACP was observed. The results showed that the activity of PAS reaction, SDH, Mg 2+ ATPase, ChE decreased markedly, and that of ACP increased obviously in starving. While in starving and exerted acute cold the activity of PAS reaction, SDH, ChE further decreased, and that of ACP and Mg 2+ ATPase increased apparently.

关 键 词：骤冷 饥饿 肝 组织化学 小鼠 

分 类 号：Q954.62[生物学—动物学]